ULCERS TREATED WITH AUXETICS 341 



iiferation of the remaining living cells, and so cause 

 the growths known as fibroids. 



The foregoing conclusions and deductions have 

 been arrived at from experimentation in vitro with 

 individual cells. As pointed out in a former chapter, 

 conclusions derived from in-vitro experimentation are 

 not in themselves sufficient to prove a point. Because 

 we can induce cell-division in individual cells on the 

 microscope stage with certain chemical agents does 

 not prove that the same division will necessarily occur 

 in vivo in the same cells in response to the same 

 agents. But, fortunately, in-vivo experimentation with 

 these agents has not been impossible, and the proof 

 that these agents, or rather some of them, do actually 

 cause proliferation in the body is now at our dis- 

 posal. In the wards of the Royal Southern Hospital 

 at Liverpool cases of chronic callous ulcers of the 

 legs were admitted, and have been treated in the first 

 instance with saturated solutions of globin. The 

 globin was applied to portions of the ulcers by dip- 

 ping pieces of sterile gauze in the solution and applying 

 it direct to the ulcerated surfaces. Granulations im- 

 mediately appeared in response. In the short space 

 of three or four hours a difference appeared between 

 the extent of the granulations in the treated as com- 

 pared with the untreated portions of the sores. In 

 twenty hours the difference was marked. Granulomata 

 have been produced in a day or two by means of 

 globin. 



Others suggested that the proliferation was not 

 necessarily due to the globin, but to the "irritation" 



