372 INTERNAL SECRETIONS OP PANCREAS AND SPLEEN. 



had been for some time removed and on others in which it 

 was prevented from dilating by ligature of its hilum at the 

 time of the experiment. He experimented in this way upon 

 a very large number of dogs and cats; nearly all his experi- 

 ments were double: i.e., performed at the same time and in 

 the same manner on two animals selected so as to resemble 

 one another as much as possible, and in only one of which had 

 the spleen been extirpated or ligatured. These experiments 

 were of two kinds: (1) those conducted with pancreatic in- 

 fusions, and (2) those carried out in the living duodenum, the 

 following being typical examples: 



"/. Infusions. Ligature of the Hilum of the Spleen. Two 

 cats, after fasting for 19 hours, received as much meat as they 

 would eat; 1 hour afterward they were etherized, and the 

 spleens, which were found to be in a state of contraction, were 

 brought out through a wound in the abdomen and their hila 

 were encircled by strong thread; in one of the animals the 

 hilum was firmly tied, but in the other it was simply encircled 

 and a knot was tied, leaving the splenic circulation perfectly 

 free (this was done in the endeavor to equalize traumatic con- 

 ditions as much as possible). The spleens were then replaced 

 in the abdominal cavity and the wound was sutured. On 

 recovering from the anesthesia the animals did not appear to 

 suffer. They were killed 6 hours later. Gastric digestion was 

 found to be more advanced in the animal in which the splenic 

 vessels were tied; the pancreas of both was cut up into small 

 fragments and infused with 100 cubic centimeters of water for 

 an hour at 35 C.; the liquid was afterward decanted and 

 returned to the warm chamber together with cubes of albumin. 



"Result. In 7 hours the pancreatic infusion of the cat in 

 which the hilum was not ligatured digested 17 grammes of 

 albumin; that of the other did not digest at all even at the 

 end of 12 hours. 



"This experiment was performed on a large number of 

 cats and dogs and always gave the same result. In spite, how- 

 ever, of the perfection of gastric digestion in the operated 

 animals, it was possible to lay at the door of traumatism the 

 absence of duodenal digestion; to correct this the experiment 

 was repeated as follows: 



