RELATIONSHIP BETWEEN SPLEEN AND PANCREAS. 373 



"Extirpation of the Spleen. Two dogs one normal, the 

 other having undergone splenectomy a month previously, but 

 at the time of the experiment in perfect health were operated 

 upon, while fasting, as follows: Etherization, ligature of the 

 pylorus, injection into the stomach, per oesophagus laid bare 

 and opened, of 50 grammes of peptone and 2 grammes of dex- 

 trin; to allow drainage of swallowed saliva the oesophagus was 

 ligatured below the opening. Both animals were killed five 

 hours later, and each pancreas was infused for three-fourths of 

 an hour in 100 cubic centimeters of water at 35 C. Although 

 death had occurred before the most favorable moment for the 

 experiment, i.e., in advance of the summit of the splenic 

 curve, the infusion coming from the dog with the spleen 

 intact digested 17 grammes of albumin in 17 hours, while the 

 other digested nothing even in 18 hours. Numerous experi- 

 ments made in this manner always gave the same result. The 

 spleenless dogs had in many cases undergone splenectomy sev- 

 eral months before the experiment, and the determination in 

 them of perfect conditions of health was always a matter of 

 great care. 



"II. Experiments in the Living Duodenum. Ligature of 

 the Duodenum at Both Ends. Two dogs after fasting for 17 

 hours received as much meat as they would eat and immedi- 

 ately afterward were operated upon as follows: Etherization, 

 laparotomy, ligature of the pylorus and of the bile-duct, intro- 

 duction into the duodenum of from 30 to 40 grammes of albu- 

 min, and ligature of the jejunal end. In one of the animals 

 the splenic hilum was also ligatured. Both were killed 7 hours 

 later. 



"Result. In the dog with the splenic hilum tied the albu- 

 min was found to be intact; it had, however, disappeared in the 

 other. 



"This experiment was also several times repeated on ani- 

 mals which had undergone splenectomy a long time previously, 

 and always yielded the same result; it is, of course, capable 

 of being combined with the preceding by making an infusion 

 of the pancreas after the death of the animals. Such infusions 

 give results in harmony with those furnished by the duodenum 

 itself. Further, it will be remembered that in the pancreatic 



