332 APPENDIX. 



1 ounce of anilin gentian- violet in dropping-bottle. 



1 ounce of Gram's solution in dropping-bottle. 



1 ounce of 30 per cent, nitric acid. 



1 ounce of xylol balsam. 



1 dozen of 13-inch filter-paper. 



1 package of blue litmus-paper. 



1 slide with concave centre. 



3 medicine-droppers. 



1 package of labels (fifty). 



1 tube of vaselin. 



Syllabus of Laboratory Work in Bacteriology. 



Explanation: The experiments presented in this syllabus 

 cover the entire requirements of the first three months' lab- 

 oratory work in bacteriology. Each student is required to 

 conduct each experiment personally, and will receive credit 

 for the experiment upon presentation of the completed work. 

 The experiments are to be conducted in the order in which 

 they are placed ; but it is not required that each be completely 

 finished before the next is begun. However, the work of 

 Part I. must be completed entirely before that of Part II. 

 can be undertaken. The details of the experiments and the 

 methods will receive explanation in the didactic course. 

 Specimens for study and material for making media will be 

 supplied from the preparation-room upon application. Each 

 student is expected to make up sufficient culture-media from 

 time to time to continue the various experiments, and it is 

 therefore taken for granted that members of the class will 

 keep themselves at all times supplied with sufficient media. 



Part I. Technique. 



EXPERIMENT 1 : Sterilization by Dry Heat : Wash and 

 dry one wire basket full of test-tubes and all of the Petri 

 saucers in the outfit. Plug the test-tubes with cotton, fit the 

 dishes together, and sterilize all by dry heat for one hour at 

 165 C. 



EXPERIMENT 2 : Culture-media : (a) Sterile Potato : Pre- 

 pare four Petri saucers with slices of potato ; also prepare 



