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APPENDIX 



LIST OF REAGENTS. 



I. REAGENTS FOR KILLING, HARDENING, PRE- 

 SERVING, AND DISSOCIATING. 



ABSOLUTE ALCOHOL. 



Use. Hardening reagent, but most used for dehydrating speci- 

 mens before clearing them in oil of cloves or xylol. 



ALCOHOL (Spirit). 



It is used of the following strengths : 90 per cent., 70 per cent., 

 50 per cent., 30 per cent. 



Preparation. 90 per cent, alcohol is the ordinary ' methy- 

 lated spirit.' The weaker solutions are obtained by 

 diluting it with water. 



Use. (1) For preserving specimens ; at least 70 per cent. 



(2) Hardening reagent ; 90 per cent. 



(3) For dissolving the intercellular substance, so that 

 the cells may be isolated by teasing ; 30 per cent. 



CHROMIC ACID, 10 per cent, solution. 



Preparation. Dissolve 10 grms. chromic acid crystals in 

 100 c.c. water. 



