494 APPENDIX. 



METHYLENE BLUE. 



Preparation. Dissolve T V grm. in 100 c.c. salt-solution. 



Use. For staining small animals in life. It brings out the 

 gland-cells, muscle-cells, and nerve elements. A small 

 quantity should be diluted with twenty times its bulk of 

 distilled water. 



METHYLENE BLUE AND EOSIN. 



Preparation. Take saturated solutions (A) of eosin and (B) of 

 methylene blue in 75 per cent, alcohol. 



Use. For staining blood-films. Take two clean cover-glasses, 

 and place a small drop in the centre of one of these. 

 Cover this with the other and after 2 seconds draw apart 

 laterally. Wave the slips in the air to dry. Dip each 

 cover-slip in (A) for 1 minute, drain rapidly, dip into 

 75 per cent, alcohol for 5 seconds and again into fresh 

 75 per cent, alcohol for 5 seconds. Then for 20 seconds 

 into solution (B). Dip momentarily into 75 per cent, 

 alcohol. Press slightly on a pad of filter paper. Wave 

 till dry. Then mount in a drop of Canada balsam placed 

 on a clean slide. 



METHYL-GREEN. 



Preparation. A saturated water-solution (8 grms. in 100 c.c.) 

 of methyl-green with a trace of 1 per cent, acetic acid. 



Use. To fix and stain the nuclei of Protozoa. 



DAHLIA, *25 per cent, solution. 



Preparation. Dissolve grm. in 100 c.c. distilled water. 

 Use. For staining Protozoa and cover-glass preparations. 



SAFBANIN. 



Preparation. Dissolve |-1 grm. in 100 c.c. water or 50 per 

 cent, alcohol. 



Use. A nuclear stain for the same objects as Dahlia. 



