[Voi*. 1 

 196 ANNALS OF THE MISSOURI BOTANICAL GARDEN 



two-thirds glycerin and one-third water at the edge of the 

 cover glass and allowing the glycerin to run under the latter as 

 the water evaporates. When concentration of the glycerin is 

 adequate, the excess should be wiped away with moist filter 

 paper and the resulting smear removed to the very edge of the 

 cover glass with a soft cloth moistened with 95 per cent alcohol. 

 The preparations may then be sealed from the atmosphere by 

 painting a ring of microscopical cement about the edge of the 

 cover glass. At least two coats should be used for this ring, a 

 light and very narrow one, and, after this dries, a very heavy, 

 broad one. I have used BelFs Microscopical Cement, made in 

 London, and Brunswick Black Cement. A variable percentage 

 of the rings crack in the course of a few years and allow the 

 glycerin to escape from under the cover glass, but the sections in 

 such preparations can be remounted. Dr. Thaxter has very 

 recently informed me that he has been using King's Transparent 

 White Cement and King's Amber Cement for fifteen years and 

 that none of the rings made with these cements have cracked. 

 By the use of circular cover glasses rather than square ones, 

 a microscopist's turn table may be used, thereby materially 

 lessening the labor of preparing the rings. 



Systematic Account 



THELEPHORACEAE 



Thelephorece Persoon, Myc. Eur. i: 109. 1822; Fries, 

 Hym. Eur. 629. 1872; Saccardo, Syll. Fung. 6:513. 1888. 



Hymenomycetes with the hymenium inferior or amphigenous 

 (on the lower surface or surrounding the fructification), cori- 

 aceous or waxy, even, rarely ribbed or papillate. 



Through several of the genera the Thelephoracece connect 

 closely with all the other families of the Hymenomycetes. 

 Hypochnus approaches Grandinia of the Hydnacece in the gran- 

 ular hymenial surface of many of the species, but can be sepa- 

 rated from this hydnaceous genus by the spore characters. 

 Lachnodadiumj with coriaceous structure, hairy stem, and 

 colorless spores, is an intermediate genus between Clavaria, of 

 the Clavariacece, and Thelephora but can be separated from the 

 latter by the spore characters. Craterellus connects with 



