After the identification of soft algae, the raw periphyton samples were cleaned of organic 

 matter using sulftiric acid, potassium dichromate, and hydrogen peroxide. Then permanent 

 diatom slides were prepared using Naphrax, a high refractive index mounting medium, following 

 Standard Methods for the Examination of Water and Wastewater (APHA 1998). At least 400 

 diatom cells (800 valves) were counted at random and identified to species. The following were 

 the main taxonomic references for the diatoms: Krammer and Lange-Bertalot 1986, 1988, 

 1991a, 1991b; Lange-Bertalot 1993, 2001; Krammer 1997a, 1997b, 2002; Reichardt 1997, 1999. 

 Diatom naming conventions followed those adopted by the Academy of Natural Sciences for 

 USGS NAWQA samples (Morales and Potapova 2000) as updated in 2003 (Dr. Eduardo 

 Morales, Academy of Natural Sciences, digital communication). Van Dam et al. (1994) was the 

 main ecological reference for the diatoms. 



The diatom proportional counts were used to generate an array of diatom association 

 metrics. A metric is a characteristic of the biota that changes in some predictable way with 

 increased human influence (Barbour et al. 1999). Diatoms are particularly useful in generating 

 metrics because there is a wealth of information available in the literature regarding the pollution 

 tolerances and water quality preferences of common diatom species (e.g., Lowe 1974, Beaver 

 1981, Lange-Bertalot 1996, Van Dam et al. 1994). 



Values for selected metrics were compared to biocriteria (numeric thresholds) developed 

 for streams in the Rocky Mountain ecoregions of Montana (Table 2). These criteria are based on 

 metric values measured in least-impaired reference streams (Bahls et al. 1992) and metric values 

 measured in streams that are known to be impaired by various sources and causes of pollution 

 (Bahls 1993). The criteria in Table 2 are vahd only for samples collected during the summer 

 field season (June 21 -September 21) and distinguish among four levels of stress or impairment 

 and three levels of aquatic life use support: (1) no impairment or only minor impairment (full 

 support); (2) moderate impairment (partial support); and (3) severe impairment (nonsupport). 

 These impairment levels correspond to excellent, good, fair, and poor biological integrity, 

 respectively. In cold, high-gradient mountain streams, natural stressors will often mimic the 

 effects of man-caused impairment on some metric values. 



