SOLVENTS AND STAINS. 



Synopsis of Treatment for Embedding Tissues and 

 for Staining and Mounting Sections. 



THE TISSUE IS PRESUMED TO BE IN ABSOLUTE 

 ALCOHOL. 



To embed in g-um. To embed in paraffin. 



1. Wash 24 to 48 hours 1. Clear 24 to 48 hours, (b) 

 in running water. 



| 2. Paraffin 1 to 4 hours. 



2. Pass into gum and syrup. 

 24 hours to several days. 3. Cut sections. 



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3. Freeze and cut sections. If unstained. If stained in 



| bulk. 



4. Wash in water to remove 4. Toluene 



gum. to remove 2. 4. Toluene or 



| | cedar oil. (b) 



5. Stain. 5. Alcohol, (a) \ 



| 5. Balsam. 



Mount in Dehydrate, (a) 6. Stain, 

 glycerine or 



Farrant. Clear, (b) 7. Dehydrate, (a) 



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Balsam. 8. Clear. 



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9. Balsam: 



(a) Absolute alcohol. 



(b) Toluene. 



A piece of tissue or a section which has been allowed to dry is spoilt. 



