34 THE SIMPLE TISSUES. 



Stain the cells. Irrigate first with water to remove the acid, 

 and then stain with hoemalum. When the staining is completed wash 

 with water, and after removal of the cover dehydrate, clear and m. B. 



Cell spaces in areolar tissue. Treat a thicker film obtained 

 as above with a drop of AgNO s sol. 1 P- C - and expose it to bright 

 sunlight, in 15 to 20 minutes a brown colour will develop. 



Examine (ff), find the irregular uncoloured cell spaces on 

 a brown ground (Schafer). 



As soon as these are evident, uncover and rinse the preparation 

 carefully with water, dehydrate and mount in balsam. 



Another preparation to bring the elastic fibres into view can be 

 made by irrigating a fresh specimen with Spiller's purple, which 

 stains them violet. (Roseanilin nitrate stains them red "Schafer.") 



Tendon. Fresh tendon. Rat's tail teased in normal saline. 

 (L) Consist of bundles of white fibrous tissue upon which (H) 

 rows of cells are indistinctly recognisable. 



Effect of acetic acid. The tissue clears and swells and the 

 cells come into view arranged in rows, each with its nucleus. 

 Very little elastic tissue is visible. 



Stain with hcemalum as above. 



Treat a fresh portion in AgNO s solution for cell spaces and outlines 

 of the epithelial tendon sheath. 



Fresh tendon from, the frog's foot may be obtained by seizing the tip 

 of a toe with strong forceps, and pulling a tendon out of the foot 

 with it. 



Rat's tail. T.S. ( p . 14 (6), s. 22 & 24, c. P., m. B.) (L) Find the 

 tendon bundles lying in grooves around the vertebrae. Recognise 

 the sheaths of fibrous tissue which surround them, and (H) the 

 tendon cells branched and deeply stained, which are dis- 

 tributed between the tendon fibres. 



Rat's tail. L.S. (Prepared as above.) Note the regular 

 arrangement of the tendon cells into rows, the position of 

 their nuclei, and the spread of their protoplasm. The pro to- 



