50 MUSCLE. 



Isolated muscular fibres of kitten, (p. 34, Morpurgo's method.) 

 A dissociated fragment of a muscle is given to you upon a 

 slide, spread a little glycerine upon it, and carefully lay the 

 fibres apart with needles, keep the fibres quite straight. 

 Examine (Lj and further dissociate until complete isolation 

 of a few fibres has been accomplished. The entire length of 

 the fibres will be seen, cover and examine (II) and sketch 

 the ends. 



Muscle for muscle spindles. T.S. Sartorius of child or small 

 animal, (p. 3, s. 22 & 24 c. P., m. B.) (L) In the Inter- 

 fascicular tisue find small blood vessels surrounded by what 

 appears as an unusual quantity of connective tissue. (H) If 

 a muscle spindle has been hit upon, the T.S. of a few muscular 

 fibres will be recognised accompanied by small blood vessels. 

 These are the presumed end organs for the muscular sense. 



Tongue of dog or cat. T.S. Coloured injection. Recognise 

 muscular fibres cut in various directions, note the longitudinally 

 seen fibres of the trans versus linguse, attached to the fibrous 

 tissue of the central "Raphe" of the tongue on the one hand, 

 and on the other to the submucous connective tissue. (H] 

 Fibres in transverse section show nuclei beneath the sarco- 

 lemma and the sarcoplasmic network between the fibrilla\ 

 This feature, not equally distinct in all fibres, is more 

 evident in young muscle. (L) The blood vessels are filled 

 with a blue coloured mass, the capillaries run parallel to 

 the muscular fibres, and are united at intervals by cross 

 branches. 



LJ RR AR N ' 



