54 NERVE. 



Nerve Cells- Multipolar nerve cells of the grey matter of 

 the Spinal Cord of a large mammal. 



Remove a small piece of the anterior horn of the grey matter of the 

 fresh cord, and compress it to a thin film hetween two cover-glasses, slip 

 them off each other and stain the films with methylene blue for one minute, 

 quickly rinse them in water and dry thoroughly in air over the bunsen 

 name, mount by inverting them when quite dry upon a drop of balsam 

 on a slide. 



(//) Find large cells stained blue, they are nucleated and their 

 protoplasm shows the chromatic patches deeply stained, and the 

 fibrillar components of the larger processes continued in 

 a radiating manner into the protoplasm. A process, the axon, 

 which does not branch is often recognisable. Other processes 

 form rich arborescent ramifications. Note the numerous nuclei 

 which belong to the neuroglia. Branching blood vessels are 

 also met with. 



