AMOEBOID LEUCOCYTES. FIBIMN. 61 



9. Dry thoroughly in the air with final warming over the 

 burner. 



10. Invert upon a drop of balsam upon a slide. 



The red cells are stained red and their nuclei, when 

 present, blue, the leucocytes have their nuclei similarly 

 coloured, and the eosinophil granules are red. 



Leucocytes of the Newt fixed in amaeboid extension. 

 A drop of newt's blood on a cover is inverted on a drop of normal saline 

 and is irrigated with the same to remove as much of the plasma as 

 possible. The preparation is then left to itself for ten minutes to give 

 the leucocytes, which have adhered to the glass, time to be in active 

 movement. The cells are next fixed (instantaneously) by heat. Play 

 a jet of steam on the cover of the preparation, which is held for two 

 seconds close to the point of issue, from a tube fitted to a flask or 

 large test-tube in which water is kept boiling briskly. Irrigate with 

 70 p>c - alcohol, followed by dilute hamiatoxj'liii for four minutes. Wash 

 away the latter with 7QP- C - alcohol, then absolute alcohol tinged with 

 eosin, followed by cedar oil and, lastly, balsam. (After Schafer. ) 



(//) Find the cells and observe the varieties of shape 

 assumed by the cytoplasm and nuclei, and the distribution of 

 the eosinophil, i.e., eosin stained granules in the former. 



Blood in Hayeni's fluid. 1 Mount a preparation made as follows : 

 Fresh blood is dropped into a considerable volume of the fluid with 

 which it must be thoroughly mixed by gentle agitation. After standing 

 from four to five hours the supernatant fluid is decanted off and the 

 blood washed with three changes of water in the same manner. Small 

 quantities are stained with picrocarmine (19) or ha?malum (22) followed 

 by eosin and mounted in glycerin or (Stirling) glycerin jelly. 



Fibrin. A thick film of blood is covered and allowed to stand 

 ten minutes. Raise the cover, rinse the adherent film of fibrin free of 

 colour with water, then invert on a drop of Spiller's purple on a slide 

 until stained (H) one minute, wash in water, dry between folds of 

 blotting paper, mount in balsam. 



^ayem's fluid. Sodium chloride lg, sodium sulph;ito 5, corrosive sublimate 

 0'5g, distilled water 200 cc. 



