114 APPENDIX TO THE HISTOLOGICAL SECTION. 



By means of this it is possible so to manage the stain as to colour 

 dividing nuclei, connective tissue fibres (blue-violet to red-violet), 

 elastic fibres (red), fibrillse of bone and Sharpey's fibres, striated 

 muscle and neuroglia of nervous tissue. 



30. Capbol-Fuchsine. Fuchsine 1, in 100 parts of a SP- C - solution 

 of phenol in water. To this add 10P- C - alcohol. 



31. Loffler's alkaline methylene blue. To a saturated alco- 

 holic solution of methylene blue 30 cc add 1 cc of a 1 P- C - solution of 

 potassium hydrate and 100 CC water. 



32. Nitrate of silver. For staining endothelial outlines a 0*2 P- C - 

 or weaker solution to 1 P- C - in distilled water is used. The surface to be 

 treated is extended without stretching (pinned out on a cork ring with 

 hedgehog bristles), is quickly rinsed with distilled water, arid then 

 flooded with the silver solution which is allowed to act for three or four 

 minutes or longer, according to the depth of staining required. Both 

 sides of a membrane may be stained. Rinse again with distilled water, 

 and place the tissue in 70P- C - alcohol and expose to sunlight. 



For demonstrating cell-spaces in tendon or connective tissue, or 

 Ranvier's crosses in nerves, exposure for 20 minutes to a 1 P- C - solution 

 will be necessary (the time depending on the light). Wash in water as 

 soon as the staining is complete and pass into balsam. For blood-vessels: 

 wash out the blood-vessels with a 2 P- C - solution of nitrate of soda, follow 

 this with an injection of a 0*2 P- C - solution of the silver salt, and inject 

 without loss of time either 70P- C - alcohol or a solution of gelatine (10 of 

 dry best gelatine in 100 cc of distilled water). Expose to sunlight. 



GolgVs chromate of silver process, for central nervous system, nerve 

 terminations, and secretory channels in glands. Tissue hardened in 

 Muller's fluid does well. A small piece of this, 4 mm , or about in. 

 thick is placed in 0*75 P- C - solution of silver nitrate for 24 to 48 hours; 

 sections are cut by hand or by the freezing method without impreg- 

 nating but simple immersion in the gum for a few minutes so as to 

 surround the preparation with gum on the plate of the microtome. 

 Mount the sections uncovered in balsam on the slide or on cover- 

 glasses, and in the latter case when the balsam is dry invert the 

 preparation on to a slide upon three feet of wax or paper, and fix 

 to the slide with a strip of gummed paper or a label with a circular 

 hole cut in it. Rapid drying of the balsam is necessary, as the 

 chromate of silver deposit soon turns granular. 



Golgi's rapid method. The fresh tissue is placed in the following 

 solution for three or four days : Potassium bichromate 3 g , 1 P- C - osmic 

 acid 30 cc , distilled water 100 CC . For each piece of 4 "^ cubed 30 cc 



