PANCREATIC DIGESTION. 143 



Pancreatic Digestion. 



The Amylolytic ferment action has already been studied 

 under starch. 



Proteolytic action. 



Make an artificial digestive fluid by adding 2 CC Liquour 

 pancreaticus (Benger) to 100 CC of a 1 p - c< solution bi-carbonate 

 of soda at 40 C. in a beaker. Add a few shreds of fibrin 

 and observe that these gradually diminish in bulk without 

 swelling (erosion). The whole process is much hastened by 

 previously soaking the fibrin in the bi-carbonate of soda, 

 but is slower than artificial gastric digestion. 



Perform the same tests as in the case of gastric digestion, 

 and note the absence of proto-proteoses. The digestion is to 

 be pushed much further than in the former case. The fluid 

 is to be filtered from coarse impurities when all the solid 

 fibrin has disappeared, and if the changes languish diluted 

 with bi-carbonate of soda solution. Note the persistent 

 bitter taste of the solution (Albumoses). After all traces of 

 albumoses have disappeared, or before, withdraw any sediment 

 which may form with a pipette and examine under the microscope 

 for leucin small yellowish balls and tyrosin colourless acicular 

 crystals. 



Whilst the above is taking place : 



Take half a tubeful of milk, add an equal volume of 

 1 p<c - solution bi-carbonate of soda and 4 drops of pancreatic 

 extract. Digest. Note the bitter taste that soon appears 

 and which may be taken as an indication of the digestive 

 change which the proteids of the milk are undergoing. 



Steaptic action fat splitting ferment. The demonstration 

 of this action does not lend itself readily to class work. 



