HAEMOGLOBIN SPECTRA. 153 



own blood to a wedgeful of water. Note in each case the 

 influence of the quantity of the pigment upon the absorption 

 bands by moving the wedge bottle across the slit of the 

 instrument. This has the same effect as dilution. 



SPECTRA OF HEMOGLOBIN COMBINED WITH GASES OR ALONE. 



1. Oxyhaemoglobin Hb0 2 . Determine the relationship of the 



two bands between D and E to the Na line and to the 

 colours. These are respectively known as the a and ft 

 bands. Compare with (3) and (8). 



NOTE : Examine the line of junction of the closed fingers 

 before a strong light, the single band of a thick solution 

 of HbO 2 will be seen. 



2. Reduced OP Gas-free Haemoglobin Hb. Add a few 



drops of (NH 4 ) 2 S or of Stokes' fluid. 1 The bands of 

 HbO 2 will gradually fuse into each other. This single 

 band is less dark than the other two. Try and restore 

 HbO 2 by shaking with air. 



3. Capbon-monoxide Hsemog-lobin-HbCO. Coal gas (4 pc CO) 



is passed for 20 minutes through blood solution. Note 

 its cherry-red colour. The two bands closely resemble 

 those of HbO 2 . Observe the relationship of the D 

 band to that line. Add (NH 4 ) 2 S there is no result. 

 This compound resists putrefaction for a long time. 



4. Neutral Met-Hsemoglobin-Met-Hb. Add 2 or 3 drops of 



a 10 p - c> solution of ferricyanide of potassium to a 

 bottleful of solution HbO 2 mix. The colour turns of 

 a brownish tint. A characteristic band appears in the 

 red and another dim band can just be recognised to 

 the green side of D. 



i Ferrous sulphate 2g, tartaric acid 3g, mix and preserve dry for use. When 

 required add 100 cc water, and add ammonia to slight alkalinity. 



