ALBUMOSURIA. H^EMOGLOBINURIA. 171 



3. Measuring tube. 



Process. Fill the measuring tube with urine to the mark U, 

 add the reagent to R, cork, and invert 10 to 12 times 

 (no shaking). Set the tube upright in a stand for 12 

 hours and read off the percentage from the graduations, 

 which give it in grammes per litre. 



The separate recognition of globulins and albumins is of no 

 importance clinically. That globulin is present may be 

 demonstrated : 



1. By dropping urine into a large quantity of water a cloud 



forms. 



2. Neutralise urine carefully, filter off any pp of phosphates, 



then add one-half its volume of a sat. solution Am 2 SO 4 , 

 the pp indicates globulin. Filter. Albumin can then be 

 demonstrated in the usual way in the filtrate. 



Albumosuria and peptonuria. See tests already given 

 under the respective substances. 



The most convenient method is to saturate some of the 

 urine with NaCl, add drops of acetic acid, boil in a beaker, 

 filter hot. The filtrate gives on cooling a pp of mixed 

 albumoses. If there be no pp, a pink Biuret reaction shows 

 pepton. 



Haemoglobirmria. Blood in urine may appear as HbO 2 , 

 Met-Hb, Acid H-tin, Hrematoporphyrin. 



Recognise these by the tests already given and especially by 

 the following : 



1. Heller's test. To the urine add one-eighth volume NaOH, 



boil ; a pp of phosphates coloured red by haematin 

 results. 



2. Guaiacum test, pg. 137. 



