174 ABNORMAL URINE. 



It is therefore necessary to proceed quickly and to delay as 

 little as possible over the earlier part of the operation, slowing 

 towards the end. Three estimations should be performed. 

 The first as rapidly as possible by running the sugar solution 

 continuously into the boiling Fehling until discoloration is 

 complete. 



From this preliminary trial is ascertained approximately 

 how much sugar solution is required to reduce all the copper. 

 In the next run this amount less 2 or 3 CC into the Fehling, 

 care being taking not to lower the temperature of the 

 Fehling below boiling by a too rapid addition. With the 

 third trial a close approximation should be attained. 



Should the sugar solution be so strong that only a few cc 

 of it reduces all the copper, then further dilution, to say 

 1 in 40, is necessary. 



Fehling '* solution. 1 cc - '05 dextrose. 



1. Dilute the urine with 19 volumes H 2 O, and place it in 

 a burette ( = 1 in 20 solution). 



2. Dilute 10 CC Fehling + 40 cc H 2 O, and place in a porce- 

 lain capsule. Keep it boiling. 



3. Run (1) into (2), until all Cu 2 O is pp and the blue 

 colour is gone. To determine this, tilt the capsule to utilise 

 the white back ground. 



4. Read off the number of cc, dilute urine used, then 

 20 : cc dilute urine used : : 1 : x = cc actual urine used. 



x contains "05 g sugar. 



100 x -05 

 To find the percentage - - = y. 



yxcc "rinem 24 hours = Quantity in 24 hours. 



