PRACTICAL PHYSIOLOGY. 



[VI. 



(a.) Dilute the solution in a test-tube and observe its spectrum, 

 noting that a stronger solution is required than with Hb0 2 , to show 



the absorption-bands. Two absorp- 

 tion-bands nearly in the same posi- 

 tion as those of Hb0 2 , but very 

 slightly nearer the violet end (fig. 24, 

 3). Make a map of the spectrum 

 and bands. 



(b.) The bands are not affected by 

 the addition of a reducing agent, 

 e.g., ammonium sulphide or Stokes's 

 fluid. Add these fluids to two 

 separate test-tubes of the solution 

 of COHb, and observe that the two 

 absorption bands are not affected 

 thereby. There is no difference on 

 C shaking the solution with air, as the 

 compound is so very stable. 



(c.) To a fresh portion of the solution of 

 carbonic oxide haemoglobin add a 10 per 

 cent, solution of caustic soda and boil = 

 cinnabar-red colour. Compare this with 

 a solution of oxy-haemoglobin similarly 

 treated. The latter gives a brownish-red 



FIG. 27. Hfematoscope of Hermann. F. / , . .., , 



Glass plate; C. Piston-like tube, () Dilute I cc. of blood with 2O cc. of 

 closed by a glass plate. By moving water + 20 cc. of caustic soda (sp. gr. I. 34). 

 C .the space BF can be increased or Ifthe b] OO( j contains CO, the fluid first 

 stTum 1 Mid%d Ck Tvess'l becomes white and cloudy, and presently 

 for holding surplus fluid. A. Sup- red. When allowed to stand, flakes form 

 port- and settle on the surface. Normal blood 



gives a dirty brown colouration. 



(e.) Non-Keduction of HbCO. Repeat the above experiment (VI. 8) with 

 carbonic oxide hemoglobin, and note that this body is not reduced by putre- 

 faction. Or seal up the blood in a tube. 



12. IV. Acid-HaBmatin. 



(a.) To diluted defibrinated blood add a few drops of glacial acetic 

 acid, and warm gently, when the mixture becomes brownish owing 

 to the formation of acid hsematin. 



(b.) The spectrum shows one absorption-band to the red side of 

 I) near C (fig. 28, 5), and there is considerable absorption of the 

 blue end of the spectrum. 



(c.) The single band is not affected by ammonium sulphide or 

 Stokes's fluid. Note that sulphur is precipitated if Am^S is used. 

 If the fluid is made alkaline hsemochromogen is formed. 



N,B. If acetic acid alone be used to effect the change, observe 

 that only one absorption-band is seen. 



