June 1902] Cultures of Uredi7ieae i?i ipoo a?id igoi 5& 



May 11, teleutospores from C. foenea (Iowa) sown on Erigeror* 

 annuus; no infection. 



May 13, teleutospores from C. foenea (Iowa) sown on Aster panic- 

 ulatus; May 20, spermogonia; May 28, aecidia. 



May 13, Teleutospores from C. foenea (Iowa) sown on Solidago- 

 canadensis ; no infection. 



May 13, teleutospores from C. foenea (Indiana) sown on Aster 

 cordifolius; May 23, spermogonia; May 30, aecidia. 



May 13, teleutospores from C. foenea (Indiana) sown on Solidago- 

 canadensis; no infection. 



PUCCINIA BOLLEYANA SACC. 



This species is first mentioned in the Amer. Mo. Micr. 

 Journal for 1889 (10:169), with an illustration but no descrip- 

 tion. It was first described in Saccardo's Sylloge (9:303), two* 

 years later. It was collected originally on a sterile sedge, pre- 

 sumably a Carex, growing from two to four feet high. The 

 type locality is within two miles of Lafayette, Ind., and only 

 an area ten or fifteen feet across supports the sedge, but almost 

 every leaf over this area has been thickly covered with the 

 rust each season since its discovery. Last year it was found in- 

 another locality about four miles distant. It has not been re- 

 ported from any other place in this or other states, but a speci- 

 men sent from Kenosha county, Wisconsin, by Dr. J. J. Davis- 

 has proved to be this species. The species is especially char- 

 acterized by the large teleutospores, and the brown, fusiform 

 uredospores. Within the last month a fruiting specimen of the 

 host has been found upon the type area, which shows it to- 

 be Carex trichocarpa Muhl. This in brief is the history of the 

 rust up to the time of making the following cultures. Whether 

 the aecidium, which has been found to grow on Sambucus cana- 

 densis, is the wide-spread Aecidium sambuci Schw., or not, it 

 would be premature to say. 



May 2, teleutospores from Carex trichocarpa sown on Sambucus- 

 canadensis ; May 10, spermogonia ; May 22, aecidia. 



May 3, teleutospores from C. trichocarpa sown on Xanthium can- 

 adense; no infection. 



May 3, teleutospores from C. trichocarpa sown on Impatiens aurea; 

 no infection. 



June 15, aecidiospores from Sambucus canadensis sown by Wm. 

 Stuart on Carex trichocarpa ; July 16, abundant uredospores first noticed^ 

 but probably not the first sori to appear. 



SUMMARY. 



During 1900 and 1901 the life cycle of the following eight 

 species of rusts was demonstrated by cultures. Of these suc- 

 cessful cultures, the first four have been previously reported, 

 while the cycle of the second four is here reported for the first 

 time. 



