17 



II BOTANICAL GAZETTE [january 



rarily grown in pots in the greenhouse. In no case was success 

 attained where definite clues derived from field observations 

 were lacking. 



Fourteen species were tried this year by the guessing 

 method, no clues being known, and the following is a record of 

 the failures, together with one instance, that of the Panicularia 

 rust, where a seeming clue also failed. Teleutospores were 

 employed in every case. 



i. Melampsora from Populus deltoides Marsh, was sown on 

 Larix europaea, Abies balsamea, Eiio?iymns obovatus, Stylophomm 

 diphyllum, Helianthus strumosus, and H. grosse-serratus, with no 

 infection. 



2. Melampsora on Salix discolor Muhl. was sown on Larix 

 europaea, Abies balsamea, Picea canadensis, Euonymus obovatus, 

 Ribes aureum, R. gracilis, and Helianthus grosse-serratus, with no 

 infection. 



3. Uromyces Junci Tul. from Juncus tenuis Willd. was sown 

 on Iris versicolor, with no infection. 



4. Uromyces Sporoboli E. & E. from Sporobolus longifolius 

 (Torr.) Wood was sown on Ceanothus america?ius and Verbena 

 stricta, with no infection. 



5. Uromyces Halstedii De T. from Homalocenchrus vir- 

 ginicus (Willd.) Britt. was sown on Senecio obovatus and Bide?is 

 frondosa, with no infection. 



6. Puccinia Eleocharidis Arth. from Eleocharis palustris 

 (L.) R. & S. was sown on Iris versicolor, with no infection. 



7. Puccinia Schedonnardi K. & S. from Schedo?inardus pan- 

 icidatus (Nutt.) Trel. was sown on Lepidium apetalum, Ceanothus 

 americanus, Oxalis cymosa, Apocynum cannabi?ium, Cassia chamae- 

 crista, Symphoricarpos racemosus, Xa?ithium canadense, Kuhnistera 

 purpurea, and Lacinaria scariosa, with no infection. 



8. Puccinia Muhlenbergiae A. & H. from Muhle?ibergia race- 

 mosa (Mx.) B. S. P. was sown on Napaea dioica, Impatie?is aurea, 

 Oxalis cymosa, Anemone canadensis and A. virginiana, Hydrophyl- 

 lum appendiculatum, Phlox divaricata, Physalodes Physalodes, Apo- 

 cynum cannabinum, and Helianthus grosse-serratus, with no infec- 

 tion. 



