1903 I 



CULTURES OF UREDIM Al. 



2Q 



period between the sowing of the dry spores and the appearance 

 of the spermogonia varying from five to seven days, and between 

 the appearance of the spermogonia and the appearance of the 

 aecidia an equal length of time, the two intervals making ten to 

 thirteen days. 



Cultures of Puccinia from Carex trichocarpa 

 on Onagra biennis. 



A collection on Carex stipata Muhl. also was made near 

 Lafayette, Indiana, spores from which were sown on Rides Cynos- 

 bati, R. floridurn, R. Uva-crispa, Aster panicidatus, A. prenanthoides, 

 Soli dago canadensis, and 5. serotina, with no infection. It was 

 not until aecidia were found in the field on seedlings of Onagra 

 biennis, growing close to the Carex still bearing dead leaves 

 covered with teleutospores, that the right plant was selected for 

 cultures. Teleutospores were sown on Onagra biennis June 23; 

 spermogonia appeared in great abundance July I, and aecidia 

 July 8. 



4. Puccinia Bolleyana Sacc. and P. Atkinsoniana Diet. 

 The connection of Puccinia Bolleyana, which occurs on Carex 

 trichocarpa Muhl., with Aecidium Sambnci Schw. has already been 

 reported by the writer. 7 This has been confirmed by further 

 successful trials. Material gathered from the type locality of 

 P. Bolleyana, when examined under the microscope, was found 

 to show only a small proportion of the characteristic spores of 



7 Jour. Myc. 8:55. 



