BOTANICAL GAZETTE [January 



io. Puccinia Helianthi Schw. Cultures showing this species 

 to be autoecious have heretofore been made with American 

 material only by Carleton 33 , but have been reported from Europe. 

 Teleutospores gathered in this vicinity on Helianthus grosse-serratus 

 Mart, were sown on the same species of host with success (cul- 

 ture dates May 8, 17, and 23) and also on H. Maximiliani Schrad. 

 (culture dates May 8, 16, and 22), but sowings at two different 

 dates on H. strumosns gave no infection. This evidence of races, 

 so far as it goes, does not accord with Carleton's opinion that 

 "there is no distinction of host forms." 



11. Phragmidium speciosum Fr. A successful culture 14 of 

 this species of rust was again tried, using teleutospores from a 

 hardy garden rose, obtained at Spirit Lake, Iowa, and sowing 

 them on Rosa humilis Marsh. The culture was made April 24, 

 and vigorous spermogonia appeared May 3, but the leaves grad- 

 ually withered without the formation of the aecidial stage. 



Beside the foregoing negative and confirmatory results, suc- 

 cessful cultures were made establishing aecidial and teleutosporic 

 association, heretofore unknown, for seven species of heteroecious 

 grass and sedge rusts. 



1. Ukomyces Aristidae E. & E. The material for the study 

 of this species was received from Mr. W. H. Long, Jr., who sug- 

 gested that its aecidial form was to be looked for on various 

 species of Plantago. Teleutospores on Aristida oligantha Michx. 

 were collected the middle of March, at Denton, Texas. Sowings 

 were made from this material on Plantago Rugelii Dec, May 2, 

 from which spermogonia arose May 13, and aecidia May 20. The 

 development was not luxuriant, but very characteristic. A sow- 

 ing on the same date on P. lanceolata, and later sowings on both 

 species gave no infection. It would seem that these two species 

 of Plantago are less suited to the ready development of the 

 species than other members of the genus. The middle of April 

 I received from Mr. Long freshly gathered aecidia on Plantago 

 virginica L., and on two undetermined species, which are doubt- 



x 3 Science 13 :25c 1901. 



14 For previous record see Bot. Gaz. 29 1271. 1900. 



