26 



1903] CULTURES OF UREDINEAE 20 



3. A second sowing was made May 27, with spermogonia June 

 3, and aecidia June 9, in equal abundance. The crumpling of 

 the leaves by the aecidia, and their bright orange color, made 

 these the most striking cultures of the season. This aecidium 

 is undoubtedly A. EllisiiTr. & Gall., founded on a collection 

 from New Mexico. 



5. Puccinia amphigena Diet. In the year 1883 the type col- 

 lection for this species was made by the writer at Ravenswood, 

 now a part of Chicago. The same season an aecidium was found 

 on Smilax herbacea in the immediate vicinity, 15 which appeared 

 to be A. Smilacis Schw. Owing to the limited distribution of 

 these two forms, and their association within a seemingly 

 restricted area, their possible genetic connection was thought 

 worth testing. This opinion was strengthened by the fact that 

 the grass rust has been collected a number of times since by the 

 writer in the same locality, but no other aecidium has been found 

 there at all likely to belong to it. 



Teleutospores on Calamovilfa longifolia (Hook.) Hack., kindly 

 sent by Rev. J. M. Bates from Callaway, Nebraska, were sown 

 May 13 on both Smilax herbacea L, and S. hispida Muhl., and on 

 May 20 both showed spermogonia, and May 31 aecidia. A sec- 

 ond sowing on May 27 was only partially successful, as the 

 leaves prematurely withered. 



6. Puccinia simillima Arth. At the time of the publication 

 of this species the writer called attention 16 to its probable con- 

 nection with an aecidium on A?iemone canadensis, which occurred 

 in great abundance in the immediate vicinity. 



Teleutospores on Phragmites Phragmites (L.) Karst. [P. com- 

 mu?iis Trim), obtained by the writer from the type locality at 

 Spirit Lake, Iowa, were sown on Anemone canadensis L., May I, 

 producing spermogonia May 5, and aecidia May 13. They were 

 also sown, partly at the same time and partly later, on Anemone 

 cylindrical A. virgmiana, Pulsatilla kirsutissima, and Ranunculus sep- 

 te?itriof talis, all seemingly reasonable hosts, but with no infection. 

 The aecidium has passed for Aecid. Ranu?iculacearum y but whether 



'5 See Burrill, Parasitic Fungi of Illinois 238. 

 i6 Bot Gaz. 34: 18. 1902. 



