m 



Jan. 1904] Cultures of Urcdineae in fpoj. 11 



Five species of rusts were successfully grown, that had been 

 studied with success before, and reported upon by the writer and 

 in part by other invesigators. Mention of them here serves to 

 confirm previous work, and in two cases to give additional knowl- 

 edge regarding hosts. 



i. iV((i.\i.\ Imiwi ikntis (Schw.) Arth. Teleutosporic 

 material of this species on Etymus Virgimcus was obtained near 

 Lafayette-, hid., and sown. May 13, on lmpaticns anrea. On May 

 iX, an abundance of spermogonia appeared, and on May 26, an 

 equal abundance of aecidia began to show. This result confirms 

 the work of last year. 3 The locality from which the teleuto- 

 sporic material was obtained for this season's work is several 

 miles from the one yielding material last year. 



2. Puccini. \ amphigena Diet. Teleutosporic material was 

 obtained by the writer on Calamovilfa longifolia from the type 

 locality at Chicago, 111. A sowing was made on a mature leaf of 

 Smilax hispida, May 23. Spermogonia appeared sparingly on 

 May 29, but were not followed by aecidia. Another sowing was 

 made on a partly grown leaf of the same host species, May 24, 

 from which a great abundance of spermogonia began to appear 

 on May 28. followed by an equal abundance of aecidia, June 

 5. Some question has been raised regarding the correctness of 

 last year's -work. 4 as the teleutosporic form is known from re- 

 gions where it is thought that Smilax does not grow, but that 

 the genuine Puccinia amphigena has its alternate form on Smilax 

 can no longer be doubted. 



3. Puccinia Andropogonis Schw. Teleutosporic material 

 on Andropogon scoparius, collected at Bloomington in southern 

 Nebraska, was sent to Rev. J. M. Bates. It was sown on Pcnt- 

 stemon kirsutus, May 19, giving rise to abundant spermogonia 

 on May 25, followed by aecidia on June 4. Previous cultures 

 have been made by Mr. William Stuart and the writer, 6 and by 

 Prof. W. A. Jvellerman. 6 There can be no question that this is 

 a wide-spread and common species in North America. 



4. Puccinia auuperidia Arth. Teleutosporic material of 

 this species on Carex gracillima was most opportunely sent from 

 Racine, Wis., by Dr. J. J. Davis. Sowing was made April 21, on 

 Ribes Cynosbati, from which abundant spermogonia appeared on 

 April 30, followed by great numbers of aecidia on May 11. A 

 sowing made at the same time on R. fioridum gave no infection. 

 Three days later a sowing made on R. aureum seemed to have 

 made a slight growth, which, nevertheless, came to naught. A 

 sowing on A'. Uva-crispa, a cut branch being placed in a glass of 



1 Bot. Gaz. J5:18. 1903. 

 4 Bot. Gaz. 35:20. 1903. 

 8 Bot. Gaz. ap:272. 1900. 

 e Jour. Mycol. 9:10. 1903. 



