3? 



Jan. 1904] Cultures oj Uredmcae in fyoj. 1 9 



sent to me in a I arch by Mr. Elam Bartholomew, from Rockport. 

 Kans. Seeds of the host were also sent, from which young plants 

 were grown for culture work. A sowing was made on April 2J ; 

 on May II, the spermogonia began to appear, and on May 18, 

 the aecidia. One later sowing was also successful, but the host 

 plants did not flourish, and the results were meager. The demon- 

 stration, however, proved ample to establish the autoecious char- 

 acter of the species. 



The aecidium of this species is so rarely seen as to give rise 

 to the conjecture that the species might not possess an aecidium. 

 The species is usually listed under Puccinia nigrescens Pk. This 

 specific name, however, belongs to the somewhat similar Euro- 

 pean species, as pointed out by Bubak, who unnecessarily be- 

 stowed the new name P. Salviac-hinccolatae upon the American 

 form. The rust occurs commonly upon the leaves, but is so much 

 more conspicuous upon the stems, especially after the leaves haye 

 partly or wholly fallen, that most collections show the caulicolous 

 form only. 



5. Uromyces on Car ex. A species of Uromyccs on Carex 

 was found. at Fair Oaks, Ind., on March 22 in very great abund- 

 ance, and in fine viable condition. The Carex grew in an open 

 sandy woodland, but in a depression of the surface where water 

 sometimes gathered during heavy rains. It grew in tufts over 

 a half acre of ground, and belonged to two species, C. lanugi- 

 nosa Michx., easily told from the abundance of last year's fruit- 

 ing culms still present, and C. varia Muhl., which showed not a 

 trace of last year's culms, and was determined from the fruiting 

 of a plant transferred to the greenhouse, and verified by a subse- 

 quent visit to the locality on May 3. On this latter visit a care- 

 ful search for aecidia was made in the vicinity of the rusted 

 ( 'arcx, but *a few young leaves of a Solidago with spermogonia 

 were the only result. These were growing with leaves inter- 

 mixed and well surrounded by the rusted Carex. This was a 

 very doubtful cine, as the common Solidago aecidium is known 

 to belong to a Puccinia, yet experience has taught that the most 

 improbable clues are not to be despised when the evidence is 

 direct. Before finding this clue, sowings of the rust on Carex 

 varia had been made on Viola Cucullaria, Isopyrum biternatum, 

 Trillium recurvatum, Ribes Cynosbati and R. auremn with no 

 infection. After the clue was obtained it was sown on five 

 species of Aster with no infection, and on five species of Solidago 

 with abundant infection. The following is the record of the 

 latter. Sown May 6 on 5. Canadensis L., showing abundant 

 spermogonia on May 14, and aecidia on May 22. A sowing May 

 26 on S. serotina Ait. gave first spermogonia June 5, and aecidia 

 June 16, the results being especially good. An equally successful 

 culture was made on each of the two hosts at subsequent dates. 

 Positive but less flourishing cultures were made on 5. ilexicaulis 



