Jan. 1904] Cultures of Uredineae in 1903. 





The collection on Cares varia Mulil.. made at Fair Oaks, 

 Ind., March 22, 1903, is taken as the type, together with the re- 

 sult of the culture on Solidago Canadensis L. obtained by a sow- 

 ing made June 3, and matured July 1, 1903, at which date it was 

 placed in the herbarium. I would tentatively refer here the collec- 

 tions referred to above on C. gracillitna, C. pubescens, C. vires- 

 cens (?) and C. lanuginosa, leaving their exact status to be deter- 

 mined later. 



The aecidium of this species does not appear to differ in 

 any marked manner from that of Puccinia Caricis-Solidaginis 

 A rth., although, perhaps, the spores are a trifle smaller. I have 

 nut. however, had opportunity of collecting it in the field, as the 

 heavy spring rains flooded the type locality and prevented all 

 subsequent development of the rust, so that in subsequent visits 

 at different times during the season the most dilligent search 

 failed to reveal any trace of it on either Solidago or Carcx. 



6. Aecidium pustulatum Curt. In early April, 1902, an 

 observation was made at Spirit Lake, Iowa, that proved very 

 puzzling for a time. On an open prairie, that had- been burned 

 over dnring the late fall, a small area showed Comandra pallida 

 A. DC. with aecidia, and in contact with it Andropogon scoparius 

 Michx. bearing uredo, of the characteristic thin-walled sort 

 known to belong to the species with aecidium on Pentstemon. 

 It was easy to find teleutospores on the grass leaves pressed into 

 hollows, thus protected from the passing fire, and only in part 

 germinated. These were collected and sown on Pentstemon 

 hirsutus with no infection. A sowing was not made on Coman- 

 dra, as no suitable growing plants were available. A similar 

 observation was made again this year at Fair Oaks, Ind., and 

 Once more under circumstances that seemed to permit of no 

 other inference but that the Comandra and Andropogon rusts 

 were connected, highly improbable as it seemed. This time 

 plants of Comandra nmbcUata (L.)'Nutt. were secured. A sow- 

 ing of teleutospores from Andropogon furcatus Muhl. was made 

 on May 5, and spermogonia began to appear on May 16, but the 

 host plant withered before time for aecidia to appear. A similar 

 sowing was made on a more vigorous host, May 25, spermo- 

 gonia appearing in great abundance May 30, and aecidia June 9. 

 Another sowing of teleutospores from A. scoparius obtained in 

 the same locality at Fair Oaks, was made on Comandra umbel- 

 lata June 1. the first spermogonia appearing June 9, and aecidia 

 June 19. Sowings of both sets of teleutospores were made twice 

 on Pentstemon hirsutus, tinder the most favorable circumstances, 

 with no infection. The conclusion is beyond all question, that 

 a common rust on species of Andropogon, not readily distin- 

 guishable from Puccinia Andropogonis Schw.. has its aecidia on 

 Comandra. being identical with Ac. pustulatum Curt. 



