51 



March 1905] Cultures of Urcdineae in 1904. 57 



gave spermogonia May 25, and aecidia May 31, in accordance 

 with results heretofore obtained. 14 



8. Puccinia Fraxinata (Schw.) Arth. Cultures of this 

 species were successfully carried out in 1899, 15 showing that the 

 aecidium occurs upon Fraxinus. As similar aecidia have been 

 collected upon Ligustrum vulgar e L., in New York, and Adelia 

 segregate, (Jacq.) Small (Forestiera porulosa Poir.) in Florida, 18 

 members of the family Oleaceae, to which Fraxinus belongs, a 

 number of trials were made to see if the number of hosts could 

 be extended. Telcutosporic material was obtained at Spirit Lake, 

 Iowa, on S parti no cynosuroides. Sowings were made on Ligus- 

 trum- vulgar e (2), Ligustrum ovali folium (2), Syringa vul- 

 garis (2), Chionanthus Virginica (2), Adelia acuminata, 

 Adelia ligustrina, and Fraxinus lanceolata, all members of the 

 family Oleaceae, yet no infection took place except on the last 

 host. Although the conditions for success were seemingly excel- 

 lent, still I am not fully convinced that some of these failures 

 were not clue to accident. I am under obligation to Dr. George 

 V. Nash, of the N. Y. Bot. Garden, for a plant of Adelia acu- 

 minata, sent from the Garden for this culture work. 



9. Puccinia Impatientis (Schw.) Arth. Teleutosporic 

 material on Elymus Virginicus was sent from Racine, Wis., by 

 Dr. J. J. Davis, and sown May 7, on Clematis Virginiana and Im- 

 patiens aurca. The first gave no infection, the second showed 

 spermogonia May 14, and aecidia May 23. 17 



10. Puccinia poculiformis (Jacq.) Wettst. Teleuto- 

 sporic material was found at Spirit Lake, Iowa, on Elymus Cana- 

 densis L., and without a careful examination was sown on Cle- 

 matis Virginiana (2), C Scottii, Cimicifuga Americana, C r ace- 

 mo sa, Actaea alba, and Impatiens aurea, with no infection. A 

 microscopic study revealed the nature of the rust, and it was 

 sown upon Berberis vulgaris May 18, with abundant infection, 

 spermogonia appearing May 25, and aecidia duly following, but 

 not recorded until June 9. Teleutosporic material was also found 

 at Spirit Lake, Iowa, on Agropyron tenerum, and was sown on 

 Berberis indgaris May 10, giving an abundant infection, first 

 showing spermogonia May 17, followed in due time by aecidia. 

 Teleutosporic material on Agropyron repens was sent from Bur- 

 lington, Vt., by Mr. William Stuart, and sown on Berberis vul- 

 garis May 11. Spermogonia appeared May 18, but the leaves 

 were injured before aecidia developed. 



14 Bot. Gaz. 29:273. 1900. and 35:1(1. 1903. 



15 Bot. Gaz. 29:275. 1900. 



ir ' Specimen in herbarium of N. Y: Bot. Garden, and cited by Sydow, 

 Monoe. Ured. 1 :808, under the erroneous name F. "paludosa". 



For previous cultures see Bot. Gaz. 35:18. 1903, and Jour. Mycol. 

 10:11. 1904. 



