V3 



March 1905] Cultures of Uredineae i?i 1904. %J *-* 59 



dissimilar to the bright orange-yellow aecidia on Ribes in the 

 fields. 



Teleutosporic material was sent by Dr. J. J. Davis from 

 Racine, Wis., on Carex gracillima, which was sown on Ribes 

 tloridum (3), R. rubrum, and R. aureum (2), with no infec- 

 tion, except that in one case a few spermogonia appeared on 

 R. aureum after eleven days, but reached no further develop- 

 ment. It was also sown with abundant success on R. Uva-crispa 

 (2), R. Cynosbati and R. rotundifolium (2), thus confirming 

 previous work.- 2 



Excellent teleutosporic material was found on Carex crinita 

 Lam., Lafayette, Ind., and sown May 7, on Ribes rubrum, 

 with no infection, on R. Uva-crispa. with weak infection, the 

 plant being in poor condition, and on R. rotundifolium with 

 strong infection, showing spermogonia May 17, and aecidia 

 May 25. Another equally strong infection was secured with 

 a sowing on the last host June 3. This culture adds another 

 teleutosporic host. 



16. Puccinia Polygoni-ampiiibii Pers. In previous 

 years 23 this rust was sown upon three species of Polygonum and 

 Cicuta macula ta with no infection. After learning of Dr. Tranz- 

 schel's successful cultures on two European species of wild 

 geranium 24 considerable effort was made to secure teleuto- 

 sporic material with which to repeat the work. No rust could 

 be found about Lafayette where it usually occurred. At Spirit 

 Lake, Iowa, a diligent search where it had previously been 

 common, only gave a few slightly rusted leaves. No better suc- 

 cess was met with at Fair Oaks, Ind. An appeal to my excel- 

 lent coadjutors, Prof. W. A. Kellerman, Columbus, Ohio, Rev. 

 J. M. Bates, Red Cloud, Neb., and Dr. J. J. Davis, Racine, 

 Wis., brought out the same experience, and only added a few 

 slightly rusted leaves from Wisconsin. The three meager col- 

 lections were, however, useless, as none of the teleutospores 

 would germinate. I then waited for aecidia to appear on ger- 

 anium and found them as rare as the teleutospores had been. 

 Finally I obtained a few belated aecidia on Geranium macula- 

 turn L. (AE. sanguinolentum Lindl.) and sowed them on 

 Polygonum emersum (Michx.) Britt., June II, and on June 

 19, uredospores appeared, and eventually on Aug. 17, teleuto- 

 spores began to form, the plants being kept in the greenhouse 

 during the culture. The American and European forms are 

 therefore identical. 



22 Jour. Mycol. 8:53. 1902, and 10:11. 1904. 



"Bot. Gaz. 35:12. 1903, and Jour. Mycol. 10:9. 1904. 



24 Centr. f. Bakt. II 2 : 106. 1903. 



