Jan. 1906] Cultures of Uredineae in 1905 64 13 



another host, were made in 1903 on six other species of hosts 

 with negative results. 5 



4. Puccinia Crandallii Pamm. & Hume, on Festuca 

 conHnis, sent from Boulder, Colo., by Mr. E. Bethel, was sown 

 on Dodecatheon Meadia, Hydrophyllum appendiculatum, Aqui- 

 legia Canadensis, Anemone Canadensis, Thalictrum dioicum, 

 Rudbeckia triloba, Gutierrezia Sarothrae, and Lonicera Japonica, 

 with no infection. 



5. Uromyces graminicola Burr, on Panicum virgatum, 

 sent from Red Cloud, Neb., by Rev. Bates, was sown on Pso- 

 ralea Onobrychis, with no infection; while similar material sent 

 by Mr. Bartholomew from Stockton, Kans., was sown on the 

 same host and also on Cassia Chamaecrista, Polemonium reptans 

 and Rudbeckia laciniata, all with no infection. 



6. Uromyces Junci (Schw.) Tul. on Junius eifusus, sent 

 by Mr. Jackson from Ithaca, N. Y., was sown on Rudbeckia 

 laciniata] R. triloba, and Falcata comosa, with no infection. 

 What is believed to be the same species of rust, but on another 

 host, was sown in 1902 on a species of Iris with negative results. 



The following species of rusts were successfully grown, and 

 the data supplement that obtained from previous cultures of this 

 series, or that published by other investigators obtained by means 

 jf similar cultures. The results in connection with Puccinia 

 Pruni-spinosae, the plum rust, are especially interesting, being 

 the first studies of the kind* with American material. 



1. Melampsora Medusae Thuem. Teleutosporic mate- 

 rial obtained near Lafayette, Ind., on Populus deltoides was sown 

 April 17 on Larix laricina and L. decidua, which resulted in 

 abundance of spermogonia appearing April 25, and a greater 

 abundance of aecidia May 1, upon both hosts. 6 



2. Gymnosporangium Juniperi-Virginianae Schw. 

 Three samples of teleutosporic material were used, one from the 

 eastern states, and two from the central west. The test was 

 designed to show whether any difference existed between the 

 two regions in the power of the species to infect the cultivated 

 apple ; the apple orchards of Iowa and the central west generally 

 being well known to be especially free from this rust. All the 

 teleutosporic material was on Juniperus Virginiana, and to all 

 appearances equally good. That sent from Durham, N. C, by 

 Mr. Wolfe, was sown on a seedling apple out of doors April 27, 

 and showed abundant spermogonia May 9, but did not develop 



5 See Jour. Myc. 10 : 10. 1904. 



8 See Jour. Mycol. 10: 13. 1904; and 11: 52. 1905. 



