65 



14 Journal of Mycology [Vol. 12 



aecidia, although conditions appeared favorable. Material from 

 Washington, Iowa, sent by Rev. Echlin, was sown April 22, on 

 a small plant of the York Imperial apple in the greenhouse, and 

 showed abundant spermogonia on May 5 ; another sowing was 

 made out of doors on a seedling apple April 27, and showed 

 spermogonia on May 13, but further observation was prevented 

 by insect depredation. A second lot of material was received 

 from Ames, Iowa, sent by Mr. Buchanan, and was sown out of 

 doors on seedling apple and Crataegus coccinea, and in the 

 .greenhouse on Amelanchier Botryapium. The sowing on apple 

 gave abundant spermogonia, but failed to make further develop- 

 ment, while on the other hosts there was no infection. So far as 

 these tests go there appears to be no difference between the east- 

 ern and western forms of the species in their power to infect the 

 cultivated apple. Many cultures have been made showing the 

 full life cycle of this rust (often called G. macropus) . A good 

 summary of the subject, especially in reference to the question 

 underlying the present work, was given a short time ago by 

 Professor Pammel 7 of the Iowa State College. 



3. Puccinia Sambuci (Schw.) Arth. Teleutosporic 

 material of fine quality on Carex lupulina, collected in Noble 

 county, near Beavor Dam, Ind., was sent by Mr. Whetzel. It 

 was sown on Sambucus Canadensis May 8, giving rise to abun- 

 dant spermogonia May 14, and aecidia May 25. 



4. Puccinia albiperidia Arth. Teleutosporic material 

 ivas gathered near Lafayette, Ind., on Carex tetanica, and sown 

 April 7 on Silphium perfoliatum, Polemonium reptans, Ambrosia 

 triUda, Rudbeckia laciniata, Steironema ciliatum, and Ribes gra- 

 <cile. Only the last host showed infection, giving abundant sper- 

 mogonia April 14, and aecidia April 27, the others remained 

 entirely free from rust. 



A part of the teleutosporic material was sent to Dr. H. Kle- 

 t>ahn, Hamburg, Germany, who sowed it on Ribes Uva-crispa, 

 R. aureum and R. rubrum. No infection resulted on R. rubrum, 

 "but on R. Uva-crispa spermogonia showed May 8, and on R. 

 aureum May 15. The further development, however, was slow, 

 soon coming to naught in the first case, and persisting much 

 longer, but with little advance in the second case. Dr. Klebahn 

 in commenting upon his results says that the imperfect develop- 

 ment of the infection on R. uva-crispa may be ascribed, in part 

 at least, to the late sowing, for the teleutospores germinated 

 freely. The host plants had already passed the most active period 

 of growth, and the weather was too warm. His results are 

 exactly in accord with our own under similar conditions. The 



7 Bull. Iowa Exper. Sta., No. 84 : 16-24. August, 1905. 



