Jan. 1906] Cultures of Uredineae i?i 1905 



66^ 



failure to secure good aecidia made it impossible to give an 

 opinion on the identity of this species of rust, 8 as compared with 

 pean forms. 



5. Puccinia Caricis-Solidaginis Arth. Teleutospores 

 mi ( arex sparganioides gathered near Lafayette, Ind., were sown 

 April 22 on Aster paniculatus and again on May 11 on A. panicu- 

 latus, A. Drummundii, Ribes rotundifolium, Urtica gracilis, and 

 Solidago Canadensis. No infection occurred except on the last 

 host, this giving spermogonia May 18 and aecidia May 28 in 

 very great abundance. In 1902 cultures of this species were 

 made with teleutospores taken from Carex Jamesii and C. stipata. 9 



6. Puccinia Peckii (DeT.) Kellerm. Teleutosporic 

 material on Carex lanuginosa, gathered at Red Cloud, Neb., by 

 Rev. Bates, was sown May 19 on Hydrophyllum appendicnlatum, 

 Steironema ciliatum, and Onagra biennis. On May 26 spermo- 

 gonia, and May 31 aecidia appeared on O. biennis, the other hosts 

 remaining entirely unaffected. This result is a duplicate of that 

 obtained in 1904. 10 . 



7. Puccinia Caricis (Schum.) Reb. Teleutosporic ma- 

 terial on Carex stipata, gathered near Lafayette, Ind., was sown 

 April 18 on Urtica gracilis, and gave spermogonia April 24, and 

 aecidia May 1, in great abundance. 



A collection in excellent condition made at Denver, Colo., 

 by Mr. Bethel, on Carex aquatilis, was sown on Urtica gracilis 

 April 10, giving few but well developed spermogonia April 18, 

 and numerous aecidia April 29. It was sown again April 25, 

 and gave abundant spermogonia May 1, followed with very 

 numerous aecidia May 8. 



Both of these sowings were tried on the evidence of the 

 microscopic examination of the collections. It was found that 

 the medium-sized teleutospores, and large uredospores found 

 intermixed, agreed with those known to belong to this species, 

 and the results confirmed the diagnosis. Both collections give 

 new hosts for the species. 11 More interesting still was the 

 presence in the Colorado collection of abundant amphispores, 

 which agree in every particular with those collected on Carex 

 stricta by C. H. Peck in New York, distributed in Thuemen's 

 Myc. Univ., No. 746, and first called Uromyces Caricis Pk., then 

 Puccinia Caricis- strictae Diet. This fortunate collection enables 

 us to show beyond a reasonable doubt, that the Uromyces Caricis 



8 For record of previous cultures see Jour. Myc. 8 : 53. 1902 ; 10 : 

 11. 1004; and 11: 58. 1905. 



9 See Bot. Gaz. 35:21. 1903. 



10 Jour. Mycol. 11 : 58. 1905. 



11 For previous cultures see Bot. Gaz. 29: 279. 1900; 55:16. 1903; 

 and Jour. Mycol. 5:52. 1902. 



