16 & Journal of Mycology [Vol. 12 



of Peck is the amphisporic form of Puccinia Caricis. What 

 determines the production of amphispores in this species is an 

 interesting question, in view of the fact that only twice have 

 they been found, and at such a great distance apart. The amphi- 

 spores in the Colorado collection did not germinate in drop cul- 

 ture, although the same conditions gave fine germination of the 

 associated teleutospores. 



8. Puccinia fraxinata (Schw.) Arth. A collection of 

 teleutospores on Spartina cynosuroides, sent by Mr. Bartholo- 

 mew from Hill City, Kans., was used to sow April 29, on Frax- 

 inus lanceolata, Adelia acuminata and Ligustrum vulgar e. Only 

 the first host gave results, showing spermogonia May 5, and 

 aecidia May 14, the sowing being made on a cut branch placed 

 in water in the greenhouse. 12 



9. Puccinia amphigena Diet. Teleutosporic material 

 on Calamovilfa longifolia sent by Rev. Bates from Red Cloud, 

 Neb., was sown on Smilax hispida May 27, and began to show 

 spermogonia June 2, and aecidia June 10, both in abundance. 18 



10. Puccinia verbenicola (E. & K.) Arth. Teleuto- 

 sporic material on Sporobohis longifolius, sent from Red Cloud, 

 Neb., by Rev. Bates, was sown on Verbena urticaefolia May 3, 

 and showed spermogonia May 9, and aecidia May 20. Another 

 collection on same host, obtained near Lafayette, Ind., was sown 

 on same species of Verbena May 27, and showed spermogonia 

 June 2, and aecidia June io. 14 



11. Puccinia pustulata (Curt.) Arth. Teleutosporic 

 material on Andropogon furcatus, gathered by the writer at Eng- 

 lish Lake, Ind., was sown on Pentstemon hirsutus and Comandra 

 umbellata May 2y, with no infection of the Pentstemon, but 

 abundant growth on the Comandra, spermogonia appearing June 

 2, and aecidia June 14. This result confirms work done in 1903. 15 



12. Puccinia Pammelii (Trel.) Arth. The cultural 

 results of 1904 16 were verified by sowing teleutospores from 

 Panicum virgatum, obtained by the writer at English Lake, Ind., 

 uponEuphorbia corcllata. A sowing was made May 26, giving 

 spermogonia June 2, and aecidia June 9. 



13. Puccinia subnitens Diet. Teleutosporic material on 

 Distichlis spicata, sent from Red Cloud, Neb., by Rev. Bates, was 



"For previous cultures see Bot. Gaz. 29 : 275. 1900; and Jour. 

 Mycol. it: 57. 1905. 



13 For previous cultures see Bot. Gaz. 55:20. 1903; and Jour Mycol. 

 10: 11. 1904. 



14 For previous cultures see Bot. Gaz. 29 : 274. 1900 ; 35 : 16. 1903 ; 

 and Jour Mycol. 11 : 56. 1905. 



15 Jour. Mycol. 10 : 17. 1904. 



16 Jour. Mycol. n: 56. 1905. 



