74 



Jan. 1906] Ctiltures of Uredineae in ipof 23 



turing, than Puccinia elegans Schrot, reported only from Argen- 

 tine, South America, an original specimen of which I have been 

 able to examine through the courtesy of Dr. P. Hennings of the 

 Botanical Garden, Berlin. P. transformans has not yet been 

 reported from any locality in the United States. 



5. Puccinia Kuhniae Schw. Teleutosporic material, 

 gathered near Lafayette, Ind., Nov. 3, 1904, on Kuhnia eupatori- 

 oides, was sown on the same host May 19. On May 28 spermo- 

 gonia appeared sparingly, which were examined under the micro- 

 scope in section, and these were followed June 5 by uredo in 

 fair abundance. The species, therefore, belongs to the group 

 of brachypuccinia. 



6. Puccinia canaliculata (Schw.) Lagerh. An obser- 

 vation in the field made in 1904 led to the present successful cul- 

 tures. Aecidia were found in remarkable abundance on seedling 

 X an tlii u m Canadense over an area 8 to 10 meters in diameter, 

 beyond which the aecidium did not occur, although the hosts 

 were equally plentiful and equally exposed. Later in the season, 

 the middle of June, uredosori were found upon what appeared 

 to be a seedling sedge extending over approximately the same 

 area that had been occupied in the spring by the cocklebur cluster 

 cups, and this was followed in August by teleutosori. Although 

 the sedge did not fruit, yet it was not difficult to ascertain that 

 it was a species of Cy perns, and the rust Puccinia canaliculata. 



On May 11 a sowing of aecidiospores, obtained from the 

 locality mentioned, -was made in the greenhouse on Cyperus 

 esculentus, other species of the genus not being at hand, and on 

 June 2 uredo were noticed, although they probably appeared ear- 

 lier and were overlooked, being small and pale. Another sow- 

 ing was made on the same species of host May 17, and uredo 

 first noticed June 2. The plants did not grow well, and the 

 infected leaves were removed for the herbarium before time 

 enough had elapsed in which to develop telutosori. Cultures with 

 teleutosporic material will doubtless confirm this association of 

 the Xanthium and Cyperus rusts. 



7. Puccinia Eleochardis Arth. Teleutosporic material 

 on Eleocharis palustris was sent by Dr. Davis from Racine, Wis., 

 and with it the information that from observations in the field 

 he believed this to have its aecidial phase on Eupatorium. Act- 

 ing upon this suggestion, a sowing was made May 5 on Eupa- 

 torium perfoliatum, which gave rise to spermogonia May 13, and 

 aecidia May 22. Another sowing on the same species of host May 

 11, gave spermogonia May 20, and aecidia May 30. Both trials 

 produced an abundance of aecidia, with all the characteristics of 

 the common and widely distributed form on this host, and closely 

 related species. 



