192 82 Journal of Mycology [Vol.13 



twice on Thalictrum dioicum. These sowings were made under 

 seemingly favorable conditions and yet no infection resulted. 

 In 1903 Dr. W. Tranzschel of St. Petersburg established the 

 connection between Puccinia P oly goni-amphibii Pers. on Poly- 

 gonum amphibium and the aecia on Geranium palustre and G. 

 pratense, and a year later the writer corroborated the discovery 

 with corresponding American species of hosts. In 1904 Dr. 

 Tranzschel showed that the rust on climbing species of Poly- 

 gonum, often included with the preceding, is distinct, either as a 

 true species or a biological species, for it produces its aecia on 

 Geranium pusillum. To see if this also could be substantiated 

 with American material the above sowings were made with seem- 

 ingly excellent teliosporic material, but the negative results leave 

 the matter an open question. The only other native Geranium 

 on which this form might be expected to grow readily is G. caro- 

 linianum, which was unfortunately not at hand for the test. 



4. Puccinia on Muhlenbergia diffusa Schreb., sent by Rev. 

 J. M. Bates from Red Cloud, Neb., was sown on Trillium recur- 

 vatum, Actaea alba, Anemonella thalictroides, Isopyrum biter- 

 natutn, Caulophyllum thalictroides, Apios Apios, Viola pubescens, 

 Dirca palustris, Althaea rosea, Callirrhoe involucrata, Napaea 

 dioica, Hibiscus Moscheutos, Marcrocalyx Nyctelea, Polemonium 

 reptans, Ambrosia trifida, and Lactuca canadensis, with no infec- 

 tion. This taken with previous trials shows that the rusts on 

 different species of Muhlenbergia are in all probability biologi- 

 cally complex. 



5. Puccinia Schedonnardi K. & S., sent by Rev. J. M. 

 Bates from Red Cloud, Neb., was sown on Callirrhoe involucrata, 

 Althaea rosea, and Ceanothus americanus, with no infection. 

 Like material from the same source was sown in 1902 on eight 

 other species of hosts with negative results. 5 The small sori 

 and fine leaves of the grass make the manipulation of material 

 of this species somewhat uncertain. 



6. Puccinia emaculata Schw. on Panicum capillare, ob- 

 tained in the vicinity of Lafayette, Ind., where it is very com- 

 mon, was sown on Polygala Senega and Napaea dioica. This 

 rust was sown in previous seasons on eighteen other species of 

 hosts. 6 



7. Uromyces on Juncus effusiis L., sent by Dr. Charles E. 

 Fairman from Ridgeway, N. Y., was sown on Polemonium rep- 

 tans, Houstonia purpurea, Ambrosia trifida, Rudbeckia laciniata, 

 Polymnia canadensis, Parthenium integrifolium, Silphium integri- 

 folium, S. perfoliatum S. terebinthinaceum, and Senecio obovatus, 



5 See Bot. Gaz. 35:11. 1903. 



6 See Bot. Gaz. 35:12. 1903; Jour. Myc. 8:52. 1902; 10 :10. 1904; 

 and 12:12. 1906. 



