194 8 4 Journal of Mycology [Vol. IS 



it would seem that what now passes under one name is either 

 a segregate, or a series of biological species, and that the Uro- 

 myces on Spartina in Indiana differs in some way from that in 

 Nebraska. 



The following species of rusts were successfully grown, and 

 the data supplement that obtained from previous cultures of 

 this series, or that recorded by other American or European in- 

 vestigators. 



i. Melampsora Bigelowii Thuem. Teliosporic material 

 obtained near Lafayette, Ind., on Salix sp., was sown April 25 

 on Larix decidua, pycnia appearing in abundance May 2, and 

 fully grown aecia about May 12. 9 



2. Cronartium Quercus (Brond.) Schroet. Work with 

 this species was suggested by Dr. C. L. Shear, who also provided 

 freshly gathered aecia on Pinus virginiana Mill., sending these a 

 number of times in varying quantity. Dr. Shear made a num- 

 ber of cultures in the open in the spring of 1905, and presented 

 a paper embodying his observations and conclusions 10 at the New 

 Orleans meeting of the American Mycological Society, but which 

 did not appear in print until June, 1906, after all data to be 

 presented here were secured. 



Aeciospores from material provided by Dr. Shear was sown 

 May 12 in the greenhouse on three plants of Quercus alba and 

 two plants of Q. velutina. On May 19 another sowing was made 

 on two other plants of Q. velutina. This work coming late in 

 the season did not receive daily examination, but on June 1 

 all the plants of Q. velutina showed uredinia, and one of them 

 also had developed telia. By June 25 the remaining four plants 

 had produced telia. The plants of Q. alba remained free from 

 infection. 



The aecia used for these cultures were the typical form of 

 Peridermium Cerebrum Peck. There seems no reason to doubt 

 the identity of the American, European and Japanese fungus,, 

 which has passed under a number of names, but a discussion of 

 the literature and facts will not be taken up in this place. 



3. Puccinia Opizii Bubak. Aecia on various wild species 

 of Lactuca, and even on the garden L. sativa, are common in 

 the extended region of the upper Mississippi valley. They were 

 described by Burrill (Bull. 111. Lab. Nat. Hist. 2:27,2. 1885), 

 but the name Aecidium compositarum Lactucae Burr, was first 

 published three years later (Saccardo, Syll. fung. 7: 799. 1888). 

 This form has often appeared in considerable abundance within 



"For previous cultures see Jour. Myc. ir :60. 1905. 

 ^ J0 Jour. Myc. 1^:89-92. 1906. 



