99 



10 Journal of Mycology [Vsl. 14 



Still a third collection made at Boulder, Colo., by the writer, was 

 sown on Hydrophyllum capitatum, Lactuca Canadensis, Iris ver- 

 sicolor, Phryma leptostachya, Artemisia serrata, and A. dracun- 

 culoides. None of these sowings gave infection. Similar mate- 

 rial had been tried on five of these hosts before, and on twenty- 

 nine other species, all with negative results. 4 The rust on this 

 host is common in the northern states east of the Rocky moun- 

 tains, and appears from its morphological characters to be distinct 

 from any described form. Fifty-five sowings have now been 

 made, beginning in 1903, using forty-three species of hosts, and 

 as no innoculation has been effected, the necessity for careful 

 field observations to detect the probable alternate host becomes 

 highly imperative in order to determine the real life cycle. 



s 



2. Puccinia on Carex gravida Bailey, sent twice by Rev. 

 J. M. Bates, first from St. Paul, Neb., was sown on Aesculus 

 glabra, Viola cucullata, Ribes Cynosbati, and Hydrophyllum 

 Virginicum, and second from Red Cloud, Neb., was sown on 

 Hypoxis erecta, Houstonia purpurea, Laciniaria spicata, Lactuca 

 Canadensis, and Artemisia dracunculoidcs, with no infection. 

 Similar material from the same region has been sown in previous 

 years upon thirty-four other species of hosts with negative 

 results. 5 



3. Puccinia on Carex Douglasii Boott. collected at Valen- 

 tine, Neb., by Rev. J. M. Bates, was sown on Symphoricarpus 

 racemosus, Napaea dioica, Psoralea Onobrychis, Aesculus glabra, 

 Laciniaria spicata, Hypoxis erecta, and Flacata comosa, with no 

 infection. 



4. Puccinia Ellisiana Thuem., on Andropogon scoparius 

 Michx., collected at Boulder, Colo., by Mr. E. Bethel, was sown 

 on Arabis sp., Thalictrum dioicum, Actaea alba, Psoralea Onobry- 

 chis, Polygala Senega, Viola striata, Polemonium reptans, Sol- 

 idago Canadensis, Boltonia asteroides, Senecio obovatus, Le- 

 pachys pinnata, and Smilacina stellata, with no infection. 



5. Puccinia virgata Ellis & Ev., on Chrysopogon avenaceus 

 (Michx.) Benth., collected at Grand Island, Neb., by Rev. J. 

 M. Bates, was sown on Ceanothus Americana, Napaea dioica, 

 Caulophyllum thalictroides, and Cassia Chamaecrista, with no 

 infection. 



* See Jour. Myc. 10 :10. 1904; 11 :51. 1905; 12 :12. 1906; and 

 75:191. 1907. 



"See Jour. Myc. 10 :10. 1904 11 :52. 1905; and 15:191. 1907. 



