16 L U O Journal of Mycology [Vol . 14 



by Dr. P. Dietel in 1892. 17 While in the foothills of Colorado 

 this spring Mr. F. D. Kern and the writer observed a plant of 

 Clematis ligustici folia with pycnia just starting, which was grow- 

 ing in the midst of some species of Agropyron, heavily coated 

 with the wintered telia of Puccinia Agropyri. Material was col- 

 lected for cultures. The host was not in fruit, but is believed 

 to be A. pseudorepens S. & S. Sowing of the spores was made 

 on Viorna Scottii May 2, sparingly giving rise to pycnia May 15, 

 but failing to develop aecia. A sowing was made May 10 on 

 Clematis Virginiana, no plants of C. ligusticifolia being available, 

 which gave rise to abundant pycnia May 20, and aecia May 27. 

 This confirms the work by Dr. Dietel, and establishes the identity 

 of the European and American form of the rust, which hereto- 

 fore rested upon purely morphological grounds. This also shows 

 that Clematis Virginiana may be a host for the species, although 

 all collections so far reported on this host pretty certainly belong 

 to the Bromus rust, Puccinia torn i para Trel. 



11. Puccinia poculiformis (Jacq.) Wettst. Teliosporic 

 material on an undetermined species of Agropyron, obtained at 

 Lake Forest, 111., by Mr. F. D. Kern and the writer, was sown 

 May 17 on Berber is vulgaris, showing pycnia May 27, and aecia 

 June 5. Another collection on Agropyron tenenim Vasey, 

 from Scotia Junction, Neb., by Rev. J. M. Bates, was sown in 

 like manner April 24, showing pycnia May 3, and aecia May 14. 



Aeciospores from the latter culture were sown on Avena 

 sativa May 25, giving rise to uredinia June 7. 18 



12. Puccinia Pammelii (Trel.) Arth. Teliosporic ma- 

 terial on Panicum zirgatum L., Collected at Red Cloud, Neb., by 

 Rev. J. M. Bates, was sown on Euphorbia corollata, with no in- 

 fection, and later was sown on E. marginata May 31, giving rise 

 to pycnia June 6, and aecia June 12. 19 



A number of attempts have been made to grow the telio- 

 spores of this rust upon Euphorbia marginata, but heretofore 

 without success. In these cases the material was collected in 

 Indiana, where E. marginata does not occur, but E. corollata is 

 common and abundant. The material used this season gives the 

 reverse condition: it was collected in Nebraska, where E. corol- 

 lata does not occur or is rare, but E. marginata is conspicuously 

 abundant. From cultures so far attempted there is reason to 

 think that we have to do with physiological species. Whether 



"Dietel, Ueber den Generationswechsel von Puccinia Agropvri Ell. 

 et Ev. Oesterr. bot. Zeitschrift 42 :261. 1892. 



18 For previous cultures see Jour. Myc. 5:53. 1902; 11 :57. 1905; 

 12:11. 1906; and jj:198. 1907. 



19 For previous cultures see Proc. Ind. Acad. Sci. for 1901 :284. 1902 ; 

 Jour. Myc. J/:56. 1905; i*:16. 1906. 



