igcM i 



Arthur: Cultures of Uredineae in 1908 d 243 



able culture material with which to test their suggestions. The 

 segregation of three additional species of western grass rusts of 

 the type of Puccinia rubigo-vera is especially gratifying. 



1. Puccinia Absintpiii DC, on Artemisia dracunculoides 

 Pursh, collected March 14, 1908, at Boulder, Colo., was sown on 

 a plant of the same host species April 9. Another collection on 

 the same host, collected March 24, 1908, at Spirit Lake, Iowa, by 

 the writer, was similarly sown April 8. Both sowings gave rise 

 to numerous pyenia first observed April 25, but doubtless appear- 

 ing fully a week sooner, and were followed by abundant uredinia 

 April 27. Owing to the early maturity of the leaves, no telia were 

 produced in either case. 



The results demonstrate that this rust has no aecial stage in its 

 life cycle. As no pyenia in connection with the uredinia had been 

 recorded for the species, either in America or Europe, and as 

 aecia on various species of Artemisia are often collected in 

 America, the status of the species had heretofore been most un- 

 certain. The situation was changed, but still unsettled, by the 

 production of aecia on Artemisia dracunculoides in the cultures 

 of last year, 37 which were grown from telia on Carex steno- 

 phylla. The present cultures very satisfactorily complete our 

 knowledge of the two most common rusts on Artemisia. 



2. Puccinia on Carex comosa Boott, collected at Lewes, Del., 

 Nov. 15, 1907, by Mr. H. S. Jackson (no. 1858), was sown April 

 25, on Iva frutescens, Urtica gracilis, and Smilax hispida, with no 

 infection, the Smilax leaves, however, having died before infec- 

 tion could have shown. Another sowing was made May 22 on 

 the same hosts, and also on Myrica cerifera and Ambrosia trifida. 

 There was no infection except in the case of Smilax, on which 

 pyenia were noticed June 8, although they might have appeared 

 sooner, and were followed by aecia June 24. 



The clues to this connection of telia and aecia were slight. In 

 reference to his collection Mr. Jackson wrote that " in the locality 

 from which the specimen came was a vine of Smilax rotundifolia 

 with aecia," but he laid little stress on this association, and did 

 not even retain a specimen of the Smilax rust. Upon visiting 

 the locality again in April no Smilax aecia could be found, doubt- 



87 See Jour. Myc. 14: 21. 1908. 



