H^ 



Arthur: Cultures of Uredixeae in io 



fluent, oblong or linear, 0.1-0.3 mm. wide by 0.5-2 mm. long, 

 tardily naked, blackish; tcliospores clavate or clavate-oblong, 13- 

 19 by 29-5 ifi, obtuse or somewhat rounded above, narrowed be- 

 low, very slightly or not constricted at the septum; wall dark 

 chestnut-brown, paler below, thin, 1-1.5/x, thicker at apex, 3-5/x, 

 smooth ; pedicel colored, short. 



On Agropyron biflorum R. & S., Lake Louise, Laggan, Alb., 

 Sept. 2, 1907, E. W. D. Holway (type) ; A. caninum (L.) Beau v., 

 Laggan, Alb., Sept. 2, 1907, E. W. D. Holway. 



8. Puccini a Muhlenbergiae Arth. & Holw. collected by Mr. 

 E. Bartholomew, March 23, 1908, at Stockton, Kans., on Muhlcn- 

 bergia glomerata Trim, was sown at different dates on ten differ- 

 ent species of host plants, including Hibiscus militaris Cav., with 

 no infection. Another collection with same data, but on M. 

 mexicana (L.) Trim, was sown April 22 on Dalea laxi flora with 

 no infection, again May 15 on same host, and on Ceanothus amcri- 

 canus and Xanthoxylum americanum, still with no infection. A 

 third attempt was made by sowing June 12, on Baptisia tinctoria, 

 Rudbeckia laciniata and Callirrhoe involucrata. Little culture 

 work is done so late in the season as this last attempt, and it was 

 supposed no infection had been secured, when on July 23 an abun- 

 dance of aecia, accompanied by a few pycnia, were noticed on the 

 plant of Callirrhoe. Although not under continuous observation, 

 it is believed they came from the sowing. 



Many attempts have been made to cultivate the rusts found on 

 various species of Muhlenbergia, twenty-six species of hosts 

 having been used. The year following the reported success by 

 Prof. W. A. Kellerman 40 in sowing rust from Muhlenbergia mex- 

 icana, collected in Ohio, on Hibiscus Moscheutos, a strong effort 

 was made to repeat his work with material from Kansas and 

 Nebraska, but with no definite result. Mr. E. Bartholomew more 

 than once suggested that the material he supplied was connected 

 with Aecidium Napaeae A. & H., as the results have seemingly 

 shown. 



The aecia obtained by Professor Kellerman, and those secured 

 by the writer, are quite unlike. The former are rather large and 



89 For record of unsuccessful sowings see Bot. Gaz. 35: 11. 1903: Jour. 

 Myc. 11: 51. 1905; and 13: 192. 1907. 

 40 Jour. Myc. 9: 109, 232. 1903. 



