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224 -* ^ Mycologia 



3. Puccinia universalis Arth., on Carex stenophylla Wahl., 

 collected at Eldorado Springs, Colo., by Mr. E. Bethel, was sown 

 April 26 on Arabis Holboclli, Sambucus canadensis, Urtica gra- 

 cilis and Artemisia dracunculoides. All remained free from in- 

 fection except the last, on which pyenia appeared May 5, and 

 aecia May 14. This is a confirmation of the result obtained in 

 1907. 15 In making the cultures and writing up the results of 

 that year it was overlooked that the combination which was then 

 established had been repeatedly suggested by Rev. J. M. Bates. 

 In the spring of 1906 Rev. Bates sent a collection of rust on 

 Car ex stenophylla, obtained at Boelus, Neb., on June 25, 1906, 

 and on the packet he wrote that it was associated with aecia on 

 Artemisia Ion gi folia. This came too late in the season to be 

 tested by a culture. In a letter received in September of the 

 same year he stated that his field observations made it almost cer- 

 tain that this Carex rust and the Artemisia aecia were alternate 

 forms of one species. Both my associate, Mr. Kern, and myself 

 were at that time of the opinion that the aecia on Artemisia be- 

 longed to the telia on the same host, and so firmly did we believe 

 this error that when material for cultures was at hand the follow- 

 ing spring, we had forgotten Rev. Bates' suggestion, and we also 

 overlooked the memorandum in our book of suggestions for 

 future work. 



4. Puccinia Caricis-Asteris Arth., on Carex f estiva Dewey, 

 the host being determined by Mr. Theo. Holm, collected August 

 15, 1908, at Granby, Colo., by Mr. E. Bethel, was sown May 17 

 in accordance with the suggestion of the collector on Aster 

 adscendens Lindl. (A. Tweed yi Rydb.), giving rise to pyenia 

 which were first noticed May 31, although they may have ap- 

 peared earlier, and to a few aecia June 6. A sowing was also 

 made at the same time on Agoseris glauca with no infection. 



Another collection made in the same vicinity five days later 

 was sown May 22 on Aster adscendens and produced abundant 

 pyenia May 29, and many aecia June 4. These cultures bring 

 forward a new set of hosts, and show the species to be of wide 

 distribution in America. 16 



15 See Jour. Myc. 14: 21. 1908. 



16 For previous cultures see Bot. Gaz. 35: 15. 1903; Jour. Myc. 8: 54- 1902; 

 and 14: 13. 1908. 



