Arthur: ( ljltures of I redinbae in [909 225 



5. PUCCINIA SUBNITENS Diet., < n Pistichlis spicata (L.) 

 Greene, collected at Kulm, \. 1).. by Dr. J. F. Brenckle, was 

 sown April 23 on Corydalis aurea Willd., with no infection! and 

 also on Atriplcx hastata. giving pyenia May 3, and aecia May u. 

 Another sowing was made April 24 on Corydalis sempervirens 

 (L.) Pers. (C. glauca Pursh), with no infection, and also on 

 Atriplcx hastata and Chcuopodium album, both of which gave 

 infection, showing pyenia April 31, and aecia later. 17 The chief 

 object in again growing this rust was to see if it would infect 

 species of Corydalis on which occur Aecidium fumariacearum 

 Kellerm. & Sw. ( . /. Corydalis Webber), the aecia and aeciospores 

 of which resemble those belonging to Puccinia subnitens. The 

 results, however, are inconclusive, and further cultures must be 

 attempted before the question can be settled. 



6. Pre (iM\ amphigena Diet., on Calamovilfa langifolia 

 (Hook.) Hack., collected at Kulm, X. D., by Dr. J. F. Brenckle, 

 was sown May 22 on Smila.v hispida, and gave numerous pyenia 

 May 31, and aecia June 4. This extends the geographical range 

 from which material has been received for successful cultures. 18 



7. Puccinia praxinata (Schw.) Arth., on Spartina cyuosu- 

 roides Willd., collected at Kulm, X. D., by Dr. J. F. Brenckle, 

 was sown May 22 on Fraxinus lanccolata, giving rise to pyenia 

 May 29, and aecia June 4. 19 



8. Puccinia Phragmitis (Schum.) Korn., on Phragmites 

 communis Trim, collected at Scotia, Xeb., by Rev. J. M. Bates, 

 was sown May 14 on Rum ex crisp us, giving rise to pyenia May 

 22, and aecia May 29, both in the greatest abundance. Another 

 collection by the same person, made at Grand Island, Neb., was 

 sown May 17 on R. crispus, giving abundance of pyenia May 23, 

 and aecia May 29. 20 



9. Puccinia obliterate Arth., on Agropyron sp., collected 



"For previous cultures see Bot. Gaz. 35: 19. 1903; Jour. Myc. n: 54. 

 1905; 12: 16. 1906; 13: 197. 1907; 14: 15. 1908; and Mycol. 1: 234. 1909. 



18 For previous cultures see Bot. Gaz. 35: 20. 1903; Jour. Myc. n : 57. 

 1905; 12: 16. 1906; and 14: 15. 1908. 



18 For previous cultures see Bot. Gaz. 29: 275. 1900; Jour. Myc. 11: 57. 

 i9 5; 12: 16. 1906; 14: 14. 1908; and Mycol. 1: 236. 1909. 



20 For previous cultures see Bot. Gaz. 29: 269. 1900; Jour. Myc. 9: 220. 

 1903 ; and 14 : 15. 1908. 



