4^4 



Arthur: Cultures oj (Jredineae i\ io^n v 229 



6rs1 showing June 12. The leaves matured so rapidly that no 

 aecia were formed. A sowing at the same date on Viola primuli- 

 folia gave no infection. This species <>f rust has been grown 

 upon Viola by Dr. John L. Sheldon,* 6 but previous attempts made 

 in my own laboratory have failed.- 7 The reason for the failures 

 seems to be the tardiness in making the cultures, by which the 

 violet leaves are too mature and ripen so rapidly that infection is 

 not secured. I am indebted to Dr. Sheldon for the suggestion-, 

 held observations and culture material, which have enabled me 

 to add this species to the list of completed life cycles. 



15. Uromyces SPARTINAE Farl., on Spartina cynosuroides 

 Willd. collected at kulm. \. 1)., by Dr. J. F. Brenckle, was 

 sown April 23 on Lysimachia quadrifolia, with no infection, and 

 at the same date on Steironema ciliatum and .V. lanceolatum, giv- 

 ing rise to pyenia May 5 on the former and May 8 on the latter. 

 In both cases growth did not extend to the formation of aecia 

 >wing to the feeble condition of the host plants. 28 The confusion 

 of this species of rust with Uromyces acuminates Arth. has 

 already been discussed above among unsuccessful cultures. 



16. Gymnosporangium globosum Farl., on Juniperus virgin- 

 tana L., collected at Asheville, X. C, by Mr. F. D. Kern, was 

 sown May 15 on Mains Iocnsis, Crataegus punctata and C. coc- 

 cinea, with no infection on the first and second, but on the last 

 giving rise to pyenia May 25 in abundance, followed by aecia 

 which were mature July 20.-'' 



17. Gymnosporangium clavipes C. & P., on Juniperus sibi- 

 rica Burgsd., collected at Leland, Mich., by Mr. F. D. Kern, was 

 sown June 5 on the fruit of Amelanchier crecta, giving rise to 

 pyenia June 14, and to mature aecia August 3, both in abundance. 

 At the same time it was sown on the leaves of Crataegus punctata. 

 giving rise to numerous pyenia, but as the plant was not well 

 established further development was soon checked. 30 There ap- 



w Torreya 9: 55. 1909. 



27 See Mycol. 1 : 232. 1909. 



28 For previous cultures see Jour. Myc. 12: 24. 1906; and 14: 17. 1908, 

 under the name U. acuminatus. 



29 For previous cultures see Jour, Myc. 13: 200. 1907; 14: 18. 1908: and 

 Mycol. 1 : 239. 1909. 



30 For previous cultures see Jour. Myc 14: 18. 1908; and Mycol. 1: 239. 

 1909. 



