&&-1.85 Mycologia 



infection only on the Euthamia, showing pycnia June 18, and 

 aecia July 5. 18 



All the hosts used in the trial are common in the vicinity where 

 the telial material was obtained and bear aecia. It is highly prob- 

 able that the aecial forms occurring on Solidago, Aster, Erigeron, 

 Euthamia, and possibly Doellingeria, belong to one species made 

 up of fairly well defined races. The form on Euthamia appears 

 from present data to constitute a race distinct from that on 

 Solidago, but is here included under the same name. 



4. Puccinia Caricis-Asteris Arth., on Carex f estiva Dewey 

 collected by Mr. E. Bethel, at Tolland, Colo., 9,000 feet altitude, 

 was sown April 13, on Aster adscendens Lindl. {A. Tweedyi 

 Rydb.), and the day following on Onagra biennis, with no infec- 

 tion on the latter and abundant infection on the former, giving 

 pycnia April 27, and aecia May 2. Another collection with same 

 data was sown on . ister adscendens April 9, and again on May 

 4, only the latter being effective, giving pycnia in abundance May 

 14, and aecia May 21. 19 



5. Puccinia Opizii Bubak, on Carex siccata Dewey, collected 

 by Dr. J. F. Brenckle, at Kulm, N. D., was sown as follows: 



April 9, Lactuca canadensis : pycnia April 16, aecia April 25. 

 April 9, Lactuca sativa: pycnia April 25, aecia May 2. 

 April 19, Lactuca canadensis: pycnia April 30, aecia none. 

 April 19, Lactuca sativa: pycnia May 6, aecia none. 



In the sowings on both dates the infection developed more 

 slowly and less abundantly on the garden lettuce (L. sativa), 

 than on the wild form. In both of the late sowings the aecia 

 failed to appear, because the leaves matured too soon and died. 20 



6. Puccinia universalis Arth., on Carex stenophylla Wahl., 

 collected by Mr. E. Bethel, at Boulder, Colo., was sown April 19, 

 on Artemisia dracunculoidcs, giving abundance of pycnia April 

 29, and aecia May 3. 21 



18 For previous cultures see Bot. Gaz. 35: 21. 1903; Jour. Myc. 12: 15. 

 1906 ; and Mycol. 1 : 233. 1909. 



19 For previous cultures see Bot. Gaz. 35: 15. 1903; Jour. Myc. 8: 54. 1902; 

 14: 13. 1908; and Mycol. 2: 224. 1910. 



20 For previous culture from an undetermined Carex see Jour. Myc. 13: 194. 

 1907. 



21 For previous cultures see Jour. Myc. 14: 21. 1908; and Mycol. 2: 224. 

 1910. 



