1* 



Mycologia 



ii. Puccinia amphigena Diet., on Calamovilfa longifolia 

 (Hook.) Hack., collected by Dr. J. F. Brenckle, at Kulm, N. D., 

 was sown April 25, on Smilax hispida, giving rise to pycnia in 

 abundance May 2, and aecia May 14. 20 



12. Puccinia Muhlenbergiae Arth. & Holw., on Muhlen- 

 bergia racemosa (Michx.) B. S. P., collected by Dr. J. F. 

 Brenckle, at Kulm, N. D., was sown May 6, on Hibiscus militaris, 

 Napaea dioica and Callirrhoe involucrata, with infection only on 

 the last, giving abundance of pycnia that were first seen May 23, 

 and aecia May 27." Another collection of the rust on M. gracilis, 

 sent by Mr. E. Bethel, from the foothills of Colorado, was sown 

 on the same three hosts, with no infection. Still a third collec- 

 tion, on M. racemosa, sent by Mr. E. Bartholomew from Stock- 

 ton, Kans., was sown May 11, on Hibiscus militaris and Althaea 

 rosea, without infection. 



13. Puccinia Rhamni (Pers.) Wettst, on Calamagrostis can- 

 adensis (Michx.) Beauv., collected by Professor W. P. Fraser, 

 at Pictou, Nova Scotia, was sown May 26, on Rhamnus alnifolia, 

 giving a strong infection, pycnia showing June 6, and aecia June 

 10. The only previous cultures in the series were made with 

 aeciospores. 28 



14. Puccinia poculiformis (Jacq.) Wettst., on Agropyron 

 tenerum Vasey, collected by Mr. E. Bethel, at Boulder, Colo., was 

 sown April 25, on Berberis vulgaris, giving pycnia May 2, and 

 aecia May 14. Another collection by Mr. Bethel, on Sitanion 

 longifolium J. G. Sm., made at Eldorado Springs, Colo., was 

 sown May 3, on Berberis vulgaris, giving pycnia May 14, and 

 aecia May 26. Still another collection on Agrostis alba L., made 

 by the writer at Isle au Haut, Me., was sown April 19, on Berberis 

 vulgaris, giving pycnia April 29, and aecia May g. 29 



15. Puccinia subnitens Diet., on Distichlis spicata (L.) 

 Greene, collected by Dr. J. F. Brenckle, at Kulm, N. D., was sown 

 May 4, on Cheno podium album, Monolepis Nuttalliana, and Cory- 



26 For previous cultures see Bot. Gaz. 35 : 20. 1903 ; Jour. Myc. 10: 11. 1004; 

 12: 16. 1906; 14: 15. 1908; and Mycol. 2: 225. 1910. 



27 For previous cultures see Mycol. 1: 251. 1909; and 2: 226. 1910. 



28 See Jour. Myc. 11: 58. 1905. 



29 For previous cultures see Jour. Myc. 8: 53. 1902; 11: 57. 1905; 12: 17. 

 1906; 13: 198. 1907; 14: 16. 1908; and Mycol. 2: 227. 1910. 



