197 



28 ^-X-^ Mycologia 



The results of these cultures agree perfectly with Aecidium 

 abundans Peck which was first collected in Colorado, on Sym- 

 phoricarpos oreophilus, the exact locality not being stated. 



2. Puccinia quadriporula Arth., on Carex Goodenovii J. Gay r 

 collected by the writer in the type locality at Isle au Haut, Me., 

 was sown April 18 on Iris versicolor, Boehmeria cylindrica, and 

 Rudbeckia laciniata, with no infection. Again April 26 it was 

 sown on Myrica cerifera, Lysimachia terrestris, Macrocalyx 

 Nyctelea, Polcmonium rep tans, Apocynum cannabinum, Senecia 

 lugens, and Aster paniculatus, with infection only on the last r 

 pycnia being first noticed May 13, and aecia appearing May 17, 

 neither very abundant. 



Field observations seemed to connect this rust with aecia on 

 Iris, but previous attempts at cultures had given no certain evi- 

 dence. 58 The results this year appear beyond question. No other 

 Carex rust grew in the vicinity of the spot where the collection 

 was made. The material used shows only the characteristic rust. 

 The aecia obtained, however, are both in gross and minute char- 

 acters indistinguishable from those of P. Caricis- Aster is Arth. 

 These facts make the status of the species enigmatical. The 

 marked diagnostic characters of P. quadriporula and P. Caricis- 

 Asteris lie in the urediniospore. The former has a somewhat 

 larger urediniospore, more usually globose, and with four, often 

 three, equatorial pores, while the latter has a smaller uredinio- 

 spore, more ellipsoid or elongated, and with two superequatorial 

 pores. The pore characters are markedly dissimilar, and without 

 intergradations. 



Another collection with the spore characters of P. quadri- 

 porula, made by Professor W. P. Fraser, at Pictou, Nova Scotia, 

 on Carex brunnescens (Pers.) Poir., was sown April n, and 

 again May 6, on Iris versicolor, Urtica gracilis, and Ribes flori- 

 dum, with no infection. 



3. Puccinia Lithospermi E. & K., on Evolvulus pilosus Nutt, 

 collected at Amarillo, Texas, by W. H. Long, was sown April 15, 

 on the same species of host, and produced a scanty infection. 

 Pycnia were not observed until May 2; aecia began t<y appear 



53 See Mycol. i : 230. 1909. 



