58 oi O Mycologia 



14. Melampsora albertensis Arth., on Popalus tremuloides 

 Michx., from Palmer Lake, Colo., sent by Mr. E. Bethel, was 

 sown April 20 on Larix laricina, Ribes Cynosbati and Pseudo- 

 tsuga mucronata, with infection only on the last, showing pycnia 

 in abundance May 4, and aecia May 9. 19 On our excursion to 

 Colorado in September Dr. Kern and the writer in company with 

 Mr. Bethel observed great areas of the mountain sides covered 

 with the yellowed foliage of P. tremuloides, almost every leaf of 

 which showed uredinia and telia of this rust. It seems remark- 

 able there should be so few collections of it in herbaria, and also 

 of its aecia on Pseudotsuga. 



15. Melampsorella elatina (A. & S.) Arth. Part of a 

 large witches' broom of Aecidium elatinum A. & S., on Abies 

 lasiocarpa Nutt, was sent by Mr. E. Bethel from Lake Eldora, 

 Colo., 9,000 feet altitude, and sown August 8 on Cerastium 

 oreophilum, giving an exceedingly abundant infection of uredinia, 

 first recorded on September 7. This is the first culture of the 

 species with American material. In Europe cultures with aecio- 

 spores have been made by von Tubeuf, 20 Klebahn, 21 and Ed. 

 Fischer, 22 and indications of races have been found. This is an 

 interesting species of rust from the unusual fact of both phases 

 having perennial mycelium. 



Successful cultures reported now for the first time. 

 The following species have never before been cultivated, in 

 America or elsewhere, so far as the writer knows. It is much to 

 be regretted that some of the species could not be brought to 

 full development, but although the results are in part imperfect, 

 they represent most important additions to previous knowledge. 



1. Puccinia Lygodesmiae Ellis & Ev., on wintered-over stems 

 of Lygodesmia juncea (Pursh) D. Don, collected April 6, 191 1, 

 by Mr. E. Bartholomew, at Stockton, Kans., was sown on plants 



19 For previous cultures see Mycol. 4: 29. 191 2. 



20 Deuts. Bot. Ges. 19: 433. 1901 ; Arb. Biol. Abth. Land.-Forstw. Kais. 

 Gesundh. 2 : 368. 



21 J ah r. Wiss. Bot. 35: 699. 1901 ; Zeits. Pfl.-Kr. 12: 139. 1902; and Jahr. 

 Hamb. Wiss. Anst. 20 3 : 31. 1902. 



^Ber. Deut. Bot. Ges. 19 : 397. 1901 ; Zeits. Pfl.-Kr. n : 321. 1901 ; and 12: 

 193. 1902. 



