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64 CtLsO Mycologia 



Negative results. It has been customary in these reports to 

 record failures to ascertain the alternate host, when sowings were 

 made with spores known to be capable of germination and made 

 upon plants that were in proper condition for infection. As the 

 work progresses, and fewer species are left to be connected with 

 alternate hosts, the need of such records diminishes, especially as 

 more and more dependence is placed upon field observations for 

 the clues to relationship. A few trials during the last three years 

 with negative results appear to be sufficiently significant to be 

 worthy of record at this time. 



i. Puccinia McClatchieana Diet. & Holw. A collection of 

 this species on Scirpus microcarpns (S. rubrotinctus) , from 

 Vaughns, Washington Co., N. Y., sent by Mr. Stewart H. Burn- 

 ham, was used for sowings made April I, 1914, on Chcione 

 glabra, Hydrophyllum virginicum, H. Fendleri, Actaea alba, Dirca 

 palnstris, and Iris versicolor, all without infection. 



This species has been confused with Puccinia angustata Peck. 

 The name was first applied to a Californian collection thought to 

 be on Scirpus sylvaiiciis, but now determined as S. microcarpus. 

 It is a common species along the Pacific coast on the last named 

 host, known in one instance as far into the mountains as Belgrade 

 near Bozeman, in southwestern Montana. It also occurs along 

 the Atlantic coast on the same host (usually called S. rubro- 

 tinctus), being known from Nova Scotia to Delaware, and inland 

 as far as Albany, N. Y. It was a collection of this species and 

 host that was cited by Peck in establishing his Puccinia angustata, 

 as on n Scirpus sylvaticum," from West Albany, N. Y. No clues 

 to the identity of the alternate host have yet been secured. 



2. Puccinia Cryptandri Ellis & Barth. A good teliosporic 

 collection of this rust on Sporobolus cryptandrus was obtained 

 by Dr. Fromme and the writer February 20, 1914, at Mesilla Park, 

 N. Mex., and sown March 20 on Sphaeralcea lobata, S. in c ana, 

 and Callirhoe digitata. Eight days later it was sown on other 

 plants of S. incana and C. digitata, and on Hibiscus militaris. 

 Again on March 9, it was sown on Hydrophyllum Fendleri, Pha- 

 celia tanacetifolia, Thalictrum Fendleri, and Abronia umbellata. 



A collection similar to the last was obtained on the preceding 

 day, but thought at the time to be Puccinia tosta, which was 



