23 2 





Arthur: Cultures of Uredinkai: ^%scll:\ 



aerial hosts to the list previously known, and further strengthens 

 the view that the species consists of a number of loosely defined 

 races. 



6. Puccinia Agropyri E. & E. Three collections on Elymus 

 canadensis from two widely separated and very different habitats 

 were successfully cultured. One collection made by Mr. E. 

 Bethel at Boulder, Colo., from plants on which he had previously 

 placed leaves of Clematis ligusticifolia covered with aecia, 

 brought from thirty miles distant, was sown April 27, on Cle- 

 matis ligusticifolia, C. Douglasii, Anemone cylindrica, Delphinium 

 Geyeri, and Thalictnim Fendleri, all believed to be probable hosts 

 of this rust, and on Phacelia heterophylla, Hydrophyllum capi- 

 tatutn, //. Fendleri, and Onosmodium occidentale, which have been 

 suspected from field observations to belong to the list of hosts. 

 Infection only occurred on Clematis ligusticifolia, showing pyenia 

 May 8, and aecia May 20. 



Another collection by Mr. Bethel from plants growing with 

 aecia-bearing Clematis lingusticifolia, at Boulder, Colo., was 

 sown May 14, on the same nine species of hosts, with infection 

 only on Clematis ligusticifolia, showing pyenia May 21, and 

 aecia May 29. 



The third collection by Dr. Brenckle from Kulm, N. Dak., was 

 sown on Clematis ligusticifolia, C. Douglasii, C. virginiana, Ane- 

 mone cylindrica, Delphinium sp. from Colorado, Thalictnim Fend- 

 leri, T. alpinum, Aquilegia caerulea, and A. flavescens, all belong- 

 ing to a quite possible group of hosts, and on the outlying 

 hosts, Phacelia heterophylla, Hydrophyllum Fendleri, Onosmod- 

 ium occidentale, and Elaeagnus argentea. Infection was obtained 

 only on Anemone cylindrica, showing a few pyenia May 20, but 

 without maturing aecia. 



Another collection of the same rust on Agropyron Smithii 

 (host determined by Prof. A. S. Hitchcock), collected by the 

 writer at Pueblo, Colo., was sown April 29, on Clematis ligustici- 

 folia, C. Douglasii, Anemone cylindrica, Delphinium sp. from Col- 

 orado, Thalictrum Fendleri, Hydrophyllum capitatum, H. Fend- 

 leri, and Onosmodium occidentale, with infection only on the first 

 named. The pyenia began to show May 8 and aecia May 17. 



