Arthur: Cultures of Uredineae 24 U s1 



on Carex vulpinoidea, at Leland, Mich., Aug. 26, 1913, was used 

 to sow April 2, on Solidago canadensis and Aster paniculatus. 

 Abundant infection occurred on the Solidago, pycnia showing 

 April 11, and aecia April 20, but the Aster remained free. The 

 collectors found in many instances old aecia on 5*. canadensis in 

 the field, intermixed with rusted Carex vulpinoidea. The infer- 

 ence from field observation, and also the cultural work of last 

 year as recorded above under number 15, is confirmed by the 

 culture. 



A collection of P. Dulichii Syd., on Dulichium arundinaceum, 

 gathered Jan. 17, 1914, at Gainesville, Fla., by Mr. H. E. Stevens, 

 was accompanied by young aecia on a species of Aster. This 

 association suggested that they might be genetically connected. A 

 sowing from the Dulichium material was made, Jan. 22, on Aster 

 Drummondii, A. paniculatus, and on an undetermined Aster, 

 which had been obtained from a field near New Orleans, La., 

 also on Solidago canadensis and Senecio obovatus. Another sow- 

 ing from the same material was made, Jan. 31, on the same three 

 species of Aster, and on A. Tweedyi. The only infection was a 

 sparing production of pycnia on Solidago canadensis, showing 

 Feb. 3, which did not continue into aecia. As soon as this result 

 was noticed, another sowing from the same material was made, 

 Feb. 4, on Solidago canadensis, which resulted in an abundant in- 

 fection, showing pycnia Feb. 16, and aecia Feb. 25. From this 

 result, together with a careful microscopic study of herbarium ma- 

 terial, it is inferred that P. Dulichii is a part of the common P. 

 cxtcnsicola, although the telial host is not a Carex. It is the first 

 time that any Carex rust has been traced to a telial host outside of 

 the genus Carex. 



20. Puccinia tosta Arth. A collection was made by Dr. 

 Fromme and the writer at Mesilla Park, N. Mex., on what was 

 thought at the time to be Muhlcnbergia Porteri, but which later 

 proved to be the very similar appearing Sporobolus asperifolius. 

 Thinking that it was P. Muhlenbergiae, known to have its aecia 

 on malvaceous hosts, it was sown, March 20, on Callirhoe invol- 

 ucrata, C. digitata, and Sphaeralcca incana. Infection was ob- 

 tained only on the last host, pycnia showing March 2J, and aecia 

 April 3, both in great profusion. Another sowing was at once 



